ISIMISO

Ulwelwesi lwedivayisi yokuhlola lumbozwe nge-microalbumin antigen endaweni yokuhlola kanye ne-antibody ye-IgG yembuzi endaweni yokulawula.Iphedi elebula limbozwe nge-fluorescence ebhalwe ukuthi microalbumin kanye ne-rabbit IgG kusengaphambili.Uma ingekho i-albumin emchameni, i-anti-Alb-ebhalwe ngegolide ene-monoclonal antibody ephepheni legolide le-colloidal izogijima kulwelwesi ngomchamo uye kulayini wokutholwa, futhi ihlanganiswe ne-Alb-coated antigen enento ebonakalayo. umugqa.Futhi umbala womugqa umnyama kunombala womugqa endaweni yokulawula(C), lokhu kuwumphumela ongemuhle.Uma umchamo uqukethe i-albumin, bazoqhudelana ne-Alb-coated antigen kulwelwesi ukuze babophezele kumasayithi amasosha omzimba alinganiselwe ku-anti-Alb-elebulwe ngegolide e-colloidal anti-monoclonal antibody.Njengoba inani le-albumin emchameni landa, ukuhlolwa

Umbala womugqa uzoba lula futhi ube lula.Okuqukethwe kwe-albumin emchameni kungatholwa ngokomthamo ngokuqhathanisa indawo yokuthola(T) nendawo yokulawula(C).Indawo yokulawula ikhwalithi (C) kanye nendawo eyinkomba (R) kukhithi izovela njalo ngesikhathi sokuhlolwa, futhi azihlanganise lutho nokuba khona kwe-albumin yomchamo.Indawo yokulawula(C) kanye nendawo eyinkomba (R) ingasetshenziswa njengenkomba yereferensi yokulawula ikhwalithi yangaphakathi yekhithi.

Inqubo Yokuhlola:

Sicela ufunde imanuwali yokusebenza kwezinsimbi kanye nokufakwa kwephakheji ngaphambi kokuhlola.Ncibilikisa amasampula ekamelweni lokushisa ngaphambi kokusetshenziswa.

1.Khipha ikhadi lokuhlola esikhwameni se-foil.Yibeke endaweni evundlile bese umaka.

2.Thatha isampula lomchamo ngepayipi elahlwayo, ulahle amathonsi amabili okuqala esampula yomchamo.Engeza amathonsi ama-3 (cishe u-100uL) omchamo ongenabhamuza phakathi nendawo yesampula yembobo yekhadi lokuhlola uqonde bese uqala ukubeka isikhathi.

3.Funda umphumela ngemizuzu eyi-10-15.Ayivumelekile uma kunemizuzu eyi-15.