Pahu Hōʻailona no Procalcitonin

(hoʻāʻo immunochromatographic fluorescence)

No ka hoʻohana ʻana i ka diagnostic in vitro wale nō

E ʻoluʻolu e heluhelu pono i kēia mea hoʻokomo i loko o ka pūʻolo ma mua o ka hoʻohana ʻana a e hahai pono i nā kuhikuhi. ʻAʻole hiki ke hōʻoia ʻia ka hilinaʻi o nā hopena hoʻāʻo inā he mau ʻokoʻa mai nā kuhikuhi i loko o kēia mea hoʻokomo i loko o ka pūʻolo.

KA HOʻOHANA I MANAʻO ʻIA

ʻO ka Diagnostic Kit no Procalcitonin (fluorescence immunochromatographic assay) he fluorescence immunochromatographic assay no ka ʻike ʻana i ka nui o Procalcitonin (PCT) i loko o ke serum kanaka a i ʻole ka plasma, hoʻohana ʻia no ka ʻike kōkua ʻana i ka maʻi bacteria a me ka sepsis. Pono e hōʻoia ʻia nā laʻana maikaʻi a pau e nā ʻano hana ʻē aʻe. Ua manaʻo ʻia kēia hoʻāʻo no ka hoʻohana ʻana o nā loea mālama ola kino wale nō.

HŌʻULUʻULU MANAʻO

Ua haku ʻia ʻo Procalcitonin me 116 mau waikawa amino a ʻo kona kaumaha molekala he 12.7KD. Hōʻike ʻia ka PCT e nā pūnaewele neuroendocrine a wāwahi ʻia e nā enzymes i loko o (immature) calcitonin, carboxy-terminating peptide, a me amino terminating peptide. He liʻiliʻi wale nō ka nui o ka PCT i loko o ke koko o ka poʻe olakino, hiki ke hoʻonui nui ʻia ma hope o ka maʻi bacteria. Ke loaʻa ka sepsis i loko o ke kino, hiki i ka hapa nui o nā ʻiʻo ke hōʻike i ka PCT, no laila hiki ke hoʻohana ʻia ka PCT ma ke ʻano he hōʻailona prognostic o ka sepsis. No kekahi mau maʻi me ka maʻi mumū, hiki ke hoʻohana ʻia ka PCT ma ke ʻano he hōʻailona o ke koho ʻana i ka antibiotic a me ka hoʻoholo ʻana i ka pono.

KUMUHANA O KE KAʻINA HANA

Ua uhi ʻia ka membrane o ka hāmeʻa hoʻāʻo me ka antibody anti PCT ma ka ʻāpana hoʻāʻo a me ka antibody IgG kao anti rabbit ma ka ʻāpana kaohi. Ua uhi ʻia ka pad lepili e ka fluorescence i kapa ʻia ʻo anti PCT antibody a me ka rabbit IgG ma mua. I ka hoʻāʻo ʻana i ka laʻana maikaʻi, hui pū ka antigen PCT i loko o ka laʻana me ka fluorescence i kapa ʻia ʻo anti PCT antibody, a hana i ka hui pale. Ma lalo o ka hana o ka immunochromatography, ke kahe nei ka paʻakikī i ke kuhikuhi o ka pepa absorbent, i ka wā i hala ai ka paʻakikī i ka ʻāpana hoʻāʻo, hui pū ʻia me ka antibody uhi anti PCT, hana i kahi paʻakikī hou. Pili maikaʻi ka pae PCT me ka hōʻailona fluorescence, a hiki ke ʻike ʻia ka nui o PCT i loko o ka laʻana e ka fluorescence immunoassay assay.