Igikoresho cyo gusuzuma kuri Procalcitonin

(fluorescence immunochromatographic assay)

Kuri vitro kwisuzumisha koresha gusa

Nyamuneka soma iyi paki shyiramo witonze mbere yo gukoresha kandi ukurikize neza amabwiriza.Ibisubizo byizewe ntibishobora kwemezwa niba hari gutandukana kwamabwiriza muriyi paki yinjizamo.

UKORESHEJWE

Igikoresho cyo kwisuzumisha kuri Procalcitonin (fluorescence immunochromatographic assay) ni fluorescence immunochromatographic assay yo kumenya umubare wa Procalcitonine (PCT) muri serumu cyangwa plasma yabantu, ikoreshwa mugupima ubufasha bwindwara ya bagiteri na sepsis.Icyitegererezo cyiza cyose kigomba kwemezwa nubundi buryo.Iki kizamini kigenewe ubuvuzi bwumwuga gusa.

INCAMAKE

Procalcitonine igizwe na aside amine 116 kandi uburemere bwa molekile ni 12.7KD.PCT igaragazwa na selile ya neuroendocrine ikagabanywa na enzymes muri calcitonine (idakuze), peptide ya carbokisi, na peptide ya amino.Abantu bafite ubuzima bwiza bafite PCT nkeya mumaraso yabo, ashobora kwiyongera cyane nyuma yo kwandura bagiteri.Iyo sepsis ibaye mumubiri, tissue nyinshi zirashobora kwerekana PCT, PCT rero irashobora gukoreshwa nkikimenyetso cyerekana ibimenyetso bya sepsis.Ku barwayi bamwe na bamwe banduye indwara, PCT irashobora gukoreshwa nk'ikimenyetso cyo guhitamo antibiyotike no guca imanza.

IHame RY'UBURYO

Ibice by'igikoresho cyo kwipimisha bisizwe na antibody anti-PCT mu karere k'ibizamini hamwe na antibody y'ihene irwanya urukwavu IgG mu karere kayobora.Lable pad yashizwemo na fluorescence yanditseho antibody ya PCT hamwe ninkwavu IgG mbere.Iyo ugerageza icyitegererezo cyiza, antigen ya PCT murugero ihuza hamwe na fluorescence yanditseho antibody ya PCT, hanyuma igakora imvange yumubiri.Mubikorwa bya immunochromatografiya, urujya n'uruza rugana mu cyerekezo cyimpapuro zinjira, iyo complexe yatsinze akarere k'ibizamini, yahujwe na anti-PCT coating antibody, ikora urwego rushya.Urwego rwa PCT rufitanye isano neza nibimenyetso bya fluorescence, kandi kwibumbira hamwe kwa PCT murugero birashobora gutahurwa na fluorescence immunoassay assay.