Kete Tohu mo te Procalcitonin

(whakamatautau immunochromatographic fluorescence)

Mo te whakamahi tātaritanga in vitro anake

Tena koa panuihia tenei kete whakauru i mua i te whakamahi me te whai i nga tohutohu.Kare e taea te whakapumau i te pono o nga hua whakamatautau mena he rereke nga tohutohu kei roto i tenei kete whakauru.

WHAKAMAHI WHAKAMAHI

Ko te Kete Tohu mo te Procalcitonin (te whakamaarama immunochromatographic fluorescence) he whakamatautau immunochromatographic fluorescence mo te kitenga ine o te Procalcitonin (PCT) i roto i te serum tangata, i te plasma ranei, ka whakamahia mo te taatai ​​awhina mo te mate huakita me te sepsis.Ko nga tauira pai katoa me whakamana e etahi atu tikanga.Ko tenei whakamatautau he tikanga mo te whakamahi ngaio hauora anake.

KAUPAPA

Ko te Procalcitonin he 116 nga waikawa amino me tona taumaha ngota he 12.7KD.Ko te PCT e whakaatuhia ana e nga pūtau neuroendocrine, ka pakaruhia e nga enzymes ki roto i te calcitonin (te pakeke), te peptide whakamutu carboxy, me te peptide whakamutu amino.He iti noa te PCT o te hunga hauora i roto i o ratou toto, ka tino piki ake i muri i te mate huakita.I te wa e pa ana te sepsis i roto i te tinana, ka taea e te nuinga o nga kopa te whakaatu i te PCT, na reira ka taea te whakamahi i te PCT hei tohu tohu mo te sepsis.Mo etahi o nga turoro he mate mumura, ka taea te whakamahi PCT hei tohu mo te kowhiringa paturopi me te whakatau whaihua.

MĀTĀPONO O TE TIKANGA

Ko te kiriuhi o te taputapu whakamatautau ka pania ki te antibody anti PCT i runga i te rohe whakamatautau me te antibody anti rabbit IgG antibody i runga i te rohe whakahaere.Ka whakakikoruatia te papa tapanga e te rewharewha kua tapaina he anti-PCT antibody me te IgG rapeti i mua.I te wa e whakamatautau ana i te tauira pai, ka hono te antigen PCT i roto i te tauira me te fluorescence kua tapaina he anti-PCT antibody, ka hangaia he ranunga aukati.I raro i te mahi o te immunochromatography, te rere matatini i roto i te ahunga o te pepa absorbent, ka pahemo matatini te rohe whakamātautau, ngā reira ki anti PCT paninga antibody, hanga matatini hou.He pai te hono o te taumata PCT ki te tohu rewharewha, a ka kitea te kukū o te PCT i roto i te tauira ma te whakamatautau i te immunoassay fluorescence.