ỤKPỤRỤ

A na-ekpuchi akpụkpọ ahụ nke ngwaọrụ nnwale ahụ na mgbochi FOB na mpaghara nnwale ahụ yana mgbochi IgG nke ewu na-alụso oke bekee na mpaghara njikwa. A na-ekpuchi paadị label ahụ site na fluorescence nke ejiri akara anti FOB na oke bekee IgG kpuchie tupu oge eruo. Mgbe a na-anwale ihe nlele dị mma, antigen FOB dị na ihe nlele ahụ na-ejikọta ya na mgbochi FOB nke ejiri akara fluorescence mee, ma mepụta ngwakọta mgbochi. N'okpuru ọrụ nke immunochromatography, usoro mgbagwoju anya na-aga n'ihu nke akwụkwọ na-amịkọrọ ihe. Mgbe mgbagwoju anya gafere mpaghara nnwale ahụ, ọ na-ejikọta ya na mgbochi FOB mkpuchi, na-emepụta mgbagwoju ọhụrụ. Ọ bụrụ na ọ dịghị mma, ihe nlele ahụ enweghị hemoglobin mmadụ ma ọ bụ ọdịnaya dị ala, enweghị ike ịmepụta mgbagwoju anya zuru oke nke ahụ ji alụso ọrịa ọgụ. Agaghị enwe ahịrị uhie na mpaghara nchọpụta (T). Ahịrị uhie bụ ọkọlọtọ na-apụta na mpaghara njikwa mma (C) iji chọpụta ma enwere ihe nlele zuru oke na ma usoro chromatography ọ bụ ihe nkịtị. A na-ejikwa ya dị ka ọkọlọtọ njikwa dị n'ime maka ihe ndị na-eme ihe.

Usoro Ule:

Biko gụọ ihe e tinyere na ngwugwu tupu ị nwalee ya.

1. Wepụ kaadị ule ahụ n'ime akpa foil ahụ, tinye ya na tebụl dị larịị wee tinye akara na ya.

2. Wepụ mkpuchi ahụ n'ime ọkpọkọ ihe nlele ahụ ma tụfuo ntapụ abụọ mbụ nke ihe nlele ahụ a gwakọtara agwakọta, tinye ntapụ atọ (ihe dị ka 100uL) nke ihe nlele ahụ a gwakọtara agwakọta nke na-enweghị afụ n'elu ya ma jiri nwayọọ nwayọọ banye n'ime olulu ihe nlele nke kaadị ahụ yana ihe nlele enyere. Mgbe ahụ, malite oge.

3. A ga-agụ ihe a chọpụtara n'ime nkeji iri ruo iri na ise, ọ gaghịkwa adị irè mgbe nkeji iri na ise gachara.