ỤKPỤRỤ

A na-eji ọgwụ mgbochi Hp-Ab kpuchie akpụkpọ ahụ nke ngwaọrụ nnwale ahụ na ọgwụ mgbochi IgG nke ewu na-alụso oke bekee n'ógbè njikwa. A na-eji fluorescence nke a na-akpọ anti Hp-Ag na rabbit IgG kpuchie mpempe akwụkwọ Lable tupu oge eruo. Mgbe a na-anwale ihe nlele dị mma, Hp-Ab dị na ihe nlele ahụ na-ejikọta ya na fluorescence nke a na-akpọ anti Hp-Ag, ma mepụta ngwakọta mgbochi. N'okpuru ọrụ nke immunochromatography, usoro mgbagwoju anya na-aga n'ihu nke akwụkwọ na-amịkọrọ ihe. Mgbe ihe mgbagwoju anya gafere mpaghara nnwale ahụ, ọ na-ejikọta ya na antibody mkpuchi anti Hp-Ag, na-emepụta mgbagwoju anya ọhụrụ. Ọ bụrụ na ọ bụ ihe na-adịghị mma, enweghị antibody HP na ihe nlele ahụ, nke mere na enweghị ike ịmepụta mgbagwoju anya mgbochi, agaghị enwe ahịrị uhie na mpaghara nchọpụta (T). Ahịrị uhie bụ ọkọlọtọ na-apụta na mpaghara njikwa mma (C) iji chọpụta ma enwere ihe nlele zuru oke na ma usoro chromatography ọ bụ ihe nkịtị. A na-ejikwa ya dị ka ọkọlọtọ njikwa dị n'ime maka ihe ndị na-eme ihe.

Usoro Ule:

Biko gụọ ihe e tinyere na ngwugwu tupu ị nwalee ya.

1. Wepụ kaadị ule ahụ n'ime akpa foil ahụ, tinye ya na tebụl dị larịị wee kaa akara na ya.

2. Tinye ntapụ abụọ nke ihe nlele serum ma ọ bụ plasma (ma ọ bụ ntapụ atọ nke ihe nlele ọbara zuru oke/mkpịsị aka) iji nwalee nke ọma nke kaadị ahụ site na ihe nlele enyere, wee tinye otu ntapụ nke ihe nlele ahụ, malite oge.

3. Chere ma ọ dịkarịa ala nkeji iri ruo iri na ise wee gụọ nsonaazụ ahụ n'ime nkeji iri ruo iri na ise. Nsonaazụ ahụ agaghị adị irè mgbe nkeji iri na ise gachara.