Matenda zida za Procalcitonin

(kuyesa kwa fluorescence immunochromatographic)

Kugwiritsa ntchito poyezetsa matenda m'thupi kokha

Chonde werengani bwino phukusili musanagwiritse ntchito ndipo tsatirani malangizo mosamala. Kudalirika kwa zotsatira za mayeso sikungatsimikizidwe ngati pali kusiyana kulikonse kuchokera ku malangizo omwe ali mu phukusili.

KUGWIRITSA NTCHITO KOFUNIKA

Chida Chodziwira Matenda cha Procalcitonin (kuyesa kwa immunochromatographic kwa kuwala) ndi njira yoyesera ya fluorescence immunochromatographic yodziwira kuchuluka kwa Procalcitonin (PCT) mu seramu yamagazi ya anthu kapena plasma, imagwiritsidwa ntchito pozindikira matenda a bakiteriya ndi sepsis. Zitsanzo zonse zabwino ziyenera kutsimikiziridwa ndi njira zina. Mayesowa ndi a akatswiri azaumoyo okha.

CHIDULE

Procalcitonin imapangidwa ndi ma amino acid 116 ndipo kulemera kwake kwa mamolekyu ndi 12.7KD. PCT imawonetsedwa ndi maselo a neuroendocrine ndipo imagawidwa ndi ma enzyme kukhala calcitonin (yosakhwima), peptide yomaliza carboxy, ndi peptide yomaliza amino. Anthu athanzi amakhala ndi PCT yochepa m'magazi awo, yomwe imatha kuwonjezeka kwambiri pambuyo pa matenda a bakiteriya. Pamene sepsis ichitika m'thupi, minofu yambiri imatha kutulutsa PCT, kotero PCT ingagwiritsidwe ntchito ngati chizindikiro cha sepsis. Kwa odwala ena omwe ali ndi matenda otupa, PCT ingagwiritsidwe ntchito ngati chizindikiro cha kusankha maantibayotiki ndi kuweruza momwe amagwirira ntchito.

MFUNDO YA NJIRAYI

Nembanemba ya chipangizo choyesera imakutidwa ndi anti-PCT antibody pamalo oyesera ndipo anti-IgG antibody ya mbuzi pamalo olamulira. Mapepala olembera amakutidwa ndi anti-PCT antibody yolembedwa ndi fluorescence ndi anti-IgG ya kalulu pasadakhale. Poyesa chitsanzo chabwino, anti-antigen ya PCT mu chitsanzo imaphatikizana ndi anti-PCT antibody yolembedwa ndi fluorescence, ndikupanga chisakanizo cha chitetezo chamthupi. Pansi pa ntchito ya immunochromatography, kuyenda kwa zovuta kumayang'ana pepala loyamwa, pamene zovutazo zidadutsa pamalo oyesera, zimaphatikizana ndi anti-PCT coating antibody, zimapanga zovuta zatsopano. Mlingo wa PCT umagwirizanitsidwa bwino ndi chizindikiro cha fluorescence, ndipo kuchuluka kwa PCT mu chitsanzo kumatha kuzindikirika ndi fluorescence immunoassay assay.