MFUNDO

Nembanemba ya chipangizo choyesera imakutidwa ndi Hp-Ab antibody pamalo oyesera ndi mbuzi anti-IgG antibody pamalo olamulira. Mapepala olembera amakutidwa ndi fluorescence yolembedwa kuti anti-Hp-Ag ndi kalulu IgG pasadakhale. Poyesa chitsanzo chabwino, Hp-Ab mu chitsanzocho imaphatikizana ndi fluorescence yolembedwa kuti anti-Hp-Ag, ndikupanga chisakanizo cha chitetezo chamthupi. Pansi pa ntchito ya immunochromatography, zovuta zimapita molunjika ku pepala loyamwa. Pamene zovutazo zidadutsa pamalo oyesera, zimaphatikizana ndi anti-Hp-Ag coating antibody, zimapanga zovuta zatsopano. Ngati zili zoipa, palibe anti-HP mu chitsanzocho, kotero kuti immune complexes sizingapangidwe, sipadzakhala mzere wofiira pamalo ozindikira (T). Mzere wofiira ndi muyezo womwe umawonekera pamalo owongolera khalidwe (C) kuti muwone ngati pali zitsanzo zokwanira komanso ngati njira ya chromatography ndi yachibadwa. Imagwiritsidwanso ntchito ngati muyezo wamkati wa ma reagents.

Njira Yoyesera:

Chonde werengani zomwe zili mu phukusi musanayese.

1. Tulutsani khadi loyesera m'thumba la foil, liyikeni patebulo lolingana ndipo liyikeni chizindikiro.

2. Onjezani madontho awiri a seramu kapena plasma (kapena madontho atatu a magazi athunthu/chala cha magazi) kuti muyese chitsime cha khadi lomwe lili ndi dispette yoperekedwa, kenako onjezerani dontho limodzi la choyezera chachitsanzo, nthawi yoyambira.

3. Dikirani kwa mphindi zosachepera 10-15 ndipo werengani zotsatira zake mu mphindi 10-15. Zotsatira zake zimakhala zosavomerezeka mutatha mphindi 15.