Agakoresho ko gusuzuma indwara ka Procalcitonin

(isuzuma rya fluorescence immunochromatographic assay)

Gukoreshwa mu gusuzuma indwara mu buryo bwa "in vitro" gusa

Mbere yo gukoresha iyi paki, soma witonze kandi ukurikize amabwiriza neza. Ntabwo bishoboka kwemeza ko ibisubizo by'isuzuma ari ukuri niba hari ibitagenda neza ku mabwiriza ari muri iyi paki.

IKORESHWA RYITEGANYANYE

Ikizamini cyo gusuzuma Procalcitonin (ikizamini cy’ubudahangarwa bw’umubiri gikozwe muri fluorescence) ni ikizamini cy’ubudahangarwa bw’umubiri gikozwe muri fluorescence kugira ngo hamenyekane Procalcitonin (PCT) mu maraso y’umuntu cyangwa mu maraso, gikoreshwa mu gusuzuma ubwandu bwa bagiteri na sepsis. Ikizamini cyose cyagaragayemo ubwandu kigomba kwemezwa n’ubundi buryo. Iki kizamini kigenewe gukoreshwa n’abaganga gusa.

INSHAMAKE

Procalcitonin igizwe na aside amine 116 kandi uburemere bwayo ni 12.7KD. PCT igaragazwa n'uturemangingo tw'imitsi y'amaraso hanyuma igacibwamo enzymes ikajyamo calcitonin (itarakura), peptide irangiza karubokisi, na peptide irangiza amino. Abantu bafite ubuzima bwiza baba bafite PCT nke mu maraso yabo, ishobora kwiyongera cyane nyuma yo kwandura bagiteri. Iyo sepsis ibaye mu mubiri, ingirangingo nyinshi zishobora kugaragaza PCT, bityo PCT ishobora gukoreshwa nk'ikimenyetso cy'uko sepsis yabaho. Ku barwayi bamwe na bamwe bafite ubwandu buterwa n'ububyimba, PCT ishobora gukoreshwa nk'ikimenyetso cy'uko imiti itoranywa n'uko ikoreshwa.

IHAME RY'UBURYO BWAYO

Agace k'igikoresho cyo gupima gatwikiriwe na antibody ya PCT ku gace k'igeragezwa naho antibody ya IgG ya antibody y'ihene ku gace k'igenzura. Udupapuro two gupima dutwikiriwe na antibody ya PCT yanditseho fluorescence na IgG ya rabbit mbere y'igihe. Mu gusuzuma icyitegererezo cyagaragaje ko ari cyiza, antigen ya PCT iri mu cyitegererezo ihuzwa na antibody ya PCT yanditseho fluorescence, maze igakora uruvange rw'ubudahangarwa. Mu gihe cy'imikorere ya immunochromatography, urujya n'uruza rw'uruvange mu cyerekezo cy'impapuro zifata, iyo complex inyuze mu gace k'igeragezwa, ihujwe na antibody ya anti PCT, ikora complex nshya. Urwego rwa PCT rufitanye isano ryiza n'ikimenyetso cya fluorescence, kandi ingano ya PCT mu cyitegererezo ishobora kumenyekana hakoreshejwe isuzuma rya fluorescence immunoassay.