Chirwere Kit kuti Procalcitonin

(kuyedza kwe fluorescence immunochromatographic)

Kushandiswa kwekuongorora in vitro chete

Ndapota verengai zviri mupakeji iyi nokungwarira musati mashandisa uye teverai mirairo yacho nemazvo. Kuvimbika kwemhedzisiro yekuyedza hakugone kuvimbiswa kana paine chero kutsauka kubva pamirairo iri mupakeji iyi.

KUSHANDISA KWAKATARISIRWA

Diagnostic Kit yeProcalcitonin (fluorescence immunochromatographic assay) inzira yekuongorora fluorescence immunochromatographic yekuona huwandu hweProcalcitonin (PCT) muropa revanhu kana muplasma, inoshandiswa pakuongorora hutachiona hwebhakitiriya uye sepsis. Sampuro dzese dzakanaka dzinofanira kusimbiswa nedzimwe nzira. Bvunzo iyi yakagadzirirwa kushandiswa nenyanzvi dzezvehutano chete.

PFUPISO

Procalcitonin inoumbwa nemaamino acids 116 uye huremu hwayo hwemamorekuru i12.7KD. PCT inoburitswa nemasero e neuroendocrine uye inoputswa nema enzymes kuita calcitonin (immature) calcitonin, carboxy-terminating peptide, uye amino terminating peptide. Vanhu vane hutano vane huwandu hushoma hwePCT muropa ravo, iyo inogona kuwedzerwa zvakanyanya mushure mehutachiona hwebhakitiriya. Kana sepsis ikaitika mumuviri, matishu mazhinji anogona kuburitsa PCT, saka PCT inogona kushandiswa sechiratidzo chekufanotaura kwe sepsis. Kune vamwe varwere vane hutachiona hwekuzvimba, PCT inogona kushandiswa sechiratidzo chekusarudza mishonga inorwisa mabhakitiriya uye kutonga kwekushanda kwayo.

NZVIMBO YEMASHURE

Kamudzira kemudziyo wekuyedza kakaputirwa neanti PCT antibody panzvimbo yekuyedza uye anti anti rabbit IgG antibody yembudzi panzvimbo yekudzora. Mapepa eLable pad akaputirwa ne fluorescence yakanyorwa anti PCT antibody uye rabbit IgG pachine nguva. Pakuyedza positive sample, PCT antigen iri mumuenzaniso inosangana ne fluorescence yakanyorwa anti PCT antibody, uye inoumba immune immune mixture. Pasi pekushanda kwe immunochromatography, complex flow inoenda kune absorbent paper, kana complex yapfuura nzvimbo yekuyedza, yakabatana ne anti PCT coating antibody, inoumba complex itsva. PCT level inoenderana zvakanaka ne fluorescence signal, uye kuwanda kwePCT mumuenzaniso kunogona kuonekwa ne fluorescence immunoassay assay.