NHEYO

Kamudzira kemudziyo wekuyedza kakaputirwa neHp-Ab antibody panzvimbo yekuyedza uye mbudzi anti rabbit IgG antibody panzvimbo yekudzora. Lable pad inoputirwa nefluorescence yakanyorwa kuti anti Hp-Ag uye rabbit IgG pachine nguva. Pakuyedza positive sample, Hp-Ab iri mu sample inosangana nefluorescence yakanyorwa kuti anti Hp-Ag, uye inoumba immune mixture. Pasi pekushanda kwe immunochromatography, complex inoyerera ichienda kune bepa rinonyudza. Kana complex yapfuura nzvimbo yekuyedza, yakabatana ne anti Hp-Ag coating antibody, inoumba complex itsva. Kana iri negative, hapana HP antibody mu sample, saka immune complexes hadzigone kuumbwa, hapazovi nemutsara mutsvuku munzvimbo yekuongororwa (T). Mutsetse mutsvuku ndiwo mwero unoonekwa munzvimbo yekudzora kunaka (C) yekutonga kana paine samples dzakakwana uye kana maitiro e chromatography ari akajairwa. Inoshandiswawo se internal control standard ye reagents.

Maitiro Ekuyedza:

Ndapota verengai zviri mupakeji musati maedza.

1. Bvisa kadhi rekuyera kubva mubhegi refoil, riise patafura yakaenzana worimaka.

2. Wedzera madonhwe maviri emushonga weropa kana weplasma (kana madonhwe matatu eropa rose/ropa remunwe) kuti utore sampuli yemushonga weropa, wobva wawedzera donhwe rimwe chete remushonga weropa, wotanga nenguva.

3. Mirira kwemaminitsi gumi kusvika gumi nemashanu wobva waverenga mhinduro yacho mumaminitsi gumi kusvika gumi nemashanu. Mhinduro yacho haishande mushure memaminitsi gumi nemashanu.