Ikhithi yokuxilonga yeProcalcitonin

(uvavanyo lwe-fluorescence immunochromatographic)

Ukusetyenziswa kokuxilongwa kwi-vitro kuphela

Nceda ufunde le phakheji ngononophelo phambi kokuba uyisebenzise kwaye ulandele imiyalelo ngokungqongqo. Ukuthembeka kweziphumo zovavanyo akunakuqinisekiswa ukuba kukho naluphi na uphambuko kwimiyalelo ekwesi sithuba sephakheji.

UKUSETYENZISWA OKUJOLISWEYO

Isixhobo sokuxilonga seProcalcitonin (uvavanyo lwe-fluorescence immunochromatographic) luvavanyo lwe-fluorescence immunochromatographic lokufumanisa ubungakanani beProcalcitonin (PCT) kwi-serum okanye kwi-plasma yomntu, isetyenziselwa ukuxilongwa kosulelo lwebhaktheriya kunye ne-sepsis. Zonke iisampulu ezilungileyo kufuneka ziqinisekiswe zezinye iindlela. Olu vavanyo lwenzelwe ukusetyenziswa ziingcali zonyango kuphela.

ISISHWANKATHELO

I-Procalcitonin yenziwe ngee-amino acids ezili-116 kwaye ubunzima bayo bemolekyuli yi-12.7KD. I-PCT ivezwa ziiseli ze-neuroendocrine kwaye yahlulwe zii-enzymes ibe yi-calcitonin (engavuthwanga), i-peptide ephelisa i-carboxy, kunye ne-peptide ephelisa i-amino. Abantu abasempilweni banenani elincinci le-PCT egazini labo, elinokwanda kakhulu emva kosulelo lwebhaktheriya. Xa i-sepsis isenzeka emzimbeni, uninzi lwezicubu zinokuveza i-PCT, ngoko ke i-PCT ingasetyenziswa njengesalathisi sokuxela kwangaphambili sepsis. Kwabanye abaguli abane-inflammatory supplementation, i-PCT ingasetyenziswa njengesalathisi sokukhethwa kwe-antibiotic kunye nokugweba ukusebenza kakuhle.

UMGAQO WENKQUBO

I-membrane yesixhobo sovavanyo igqunywe nge-anti-PCT antibody kwindawo yovavanyo kunye ne-anti-rabbit IgG antibody yebhokhwe kwindawo yokulawula. I-Lable pad igqunywe nge-fluorescence ebhalwe nge-anti-PCT antibody kunye ne-rabbit IgG kwangaphambili. Xa kuvavanywa isampuli elungileyo, i-PCT antigen kwisampuli idibana ne-anti-PCT antibody ebhalwe nge-fluorescence, kwaye yenze umxube womzimba. Phantsi kwesenzo se-immunochromatography, ukuhamba okuntsonkothileyo kwicala lephepha elifunxayo, xa i-complex idlula kwindawo yovavanyo, idibene ne-anti-PCT coating antibody, yenza i-complex entsha. Inqanaba le-PCT lihambelana kakuhle nesignali ye-fluorescence, kwaye uxinzelelo lwe-PCT kwisampuli lunokufunyanwa ngovavanyo lwe-fluorescence immunoassay.