ÌLÀNÀ

A fi antibody Hp-Ab bo awọ ara ẹrọ idanwo naa ni agbegbe idanwo ati antibody IgG antirabbit lori agbegbe iṣakoso. A fi fluorescence ti a samisi anti Hp-Ag ati ehoro IgG bo paadi Lable ni ilosiwaju. Nigbati a ba n dan ayẹwo ayẹwo rere, Hp-Ab ninu ayẹwo naa darapọ mọ fluorescence ti a samisi anti Hp-Ag, wọn si ṣe adalu ajẹsara. Labẹ iṣẹ immunochromatography, sisan eka naa wa ni itọsọna ti iwe gbigba. Nigbati complex ba kọja agbegbe idanwo naa, o darapọ mọ antibody ti a bo anti Hp-Ag, o ṣẹda complex tuntun. Ti o ba jẹ odi, ko si antibody HP ninu ayẹwo naa, nitorinaa awọn complex ajẹsara ko le ṣe agbekalẹ, ko si ila pupa ni agbegbe wiwa (T). Laini pupa ti o jẹ boṣewa naa han ni agbegbe iṣakoso didara (C) lati ṣe idajọ boya awọn ayẹwo to wa ati boya ilana chromatography jẹ deede. A tun lo o gẹgẹbi boṣewa iṣakoso inu fun awọn reagents.

Ilana Idanwo:

Jọwọ ka ohun ti a fi sinu apo naa ṣaaju idanwo naa.

1. Yọ kaadi idanwo kuro ninu apo foil naa, fi si ori tabili ipele naa ki o si samisi i.

2. Fi omi ìṣàn ...

3. Duro fun o kere ju iṣẹju 10-15 ki o ka abajade naa laarin iṣẹju 10-15. Abajade naa ko ni ibamu lẹhin iṣẹju 15.