KA'IDA

An rufe membrane na na'urar gwajin da haɗin BSA da 25-(OH)VD a yankin gwaji da kuma antibody na IgG na maganin goat akan yankin sarrafawa. Ana shafa faifan alamar da alamar haske ta antibody na 25-(OH)VD da kuma IgG na zomo a gaba. Lokacin gwada samfurin, 25-(OH)VD a cikin samfurin yana haɗuwa da antibody mai alamar haske ta anti 25-(OH)VD, kuma yana samar da cakuda garkuwar jiki. A ƙarƙashin aikin immunochromatography, kwararar hadaddun a cikin alkiblar takardar sha, lokacin da hadaddun ya wuce yankin gwaji, za a haɗa alamar fluorescent kyauta tare da 25-(OH)VD akan membrane. Yawan 25-(OH)VD yana da alaƙa mara kyau ga siginar fluorescent, kuma yawan 25-(OH)VD a cikin samfurin ana iya gano shi ta hanyar gwajin immunoassay na fluorescence.

Tsarin Gwaji:

Da fatan za a karanta littafin aikin kayan aiki da kuma abin da aka saka a cikin fakitin kafin a gwada.

1. A ajiye dukkan sinadaran da aka haɗa da samfuran a zafin ɗaki.
2. Buɗe Mai Nazarin Jiki Mai Ɗaukewa (WIZ-A101), shigar da kalmar sirri ta asusun bisa ga hanyar aiki ta kayan aikin, sannan ka shigar da hanyar ganowa.
3. Duba lambar haƙoran don tabbatar da abin gwajin.
4. Cire katin gwaji daga jakar foil.
5. Saka katin gwaji a cikin ramin katin, duba lambar QR, sannan ka tantance abin gwajin.
6. Sai a zuba 15μL na serum ko samfurin plasma a cikin ruwan, sannan a gauraya sosai
7. A zuba hadin 80μL don a yi gwajin rijiyar katin.
8. Danna maɓallin "gwaji na yau da kullun", bayan mintuna 15, kayan aikin zai gano katin gwaji ta atomatik, zai iya karanta sakamakon daga allon nuni na kayan aikin, kuma ya yi rikodin/buga sakamakon gwajin.
9. Duba umarnin Portable Immune Analyzer (WIZ-A101).