MFUNDO

Nembanemba ya chipangizo choyesera imakutidwa ndi conjugate ya BSA ndi 25-(OH) VD pagawo loyeserera ndi anti-anti rabbit IgG antibody pagawo lowongolera.Marker pad amakutidwa ndi fluorescence mark anti 25-(OH) VD antibody ndi kalulu IgG pasadakhale.Mukayesa zitsanzo, 25-(OH) VD mu zitsanzo phatikizani ndi fluorescence zolembedwa anti 25-(OH)VD antibody, ndi kupanga osakaniza chitetezo.Pansi pa zochita za immunochromatography, kuyenda movutikira kunjira ya pepala loyamwa, pamene zovuta zidadutsa gawo loyesa, Chizindikiro cha fulorosenti yaulere chidzaphatikizidwa ndi 25-(OH) VD pa nembanemba.Kuphatikizika kwa 25-(OH) VD ndikulumikizana koyipa kwa siginecha ya fluorescence, ndipo kuchuluka kwa 25-(OH) VD mu zitsanzo kumatha kuzindikirika ndi fluorescence immunoassay assay.

Njira Yoyesera:

Chonde werengani buku lothandizira zida ndi kuyika phukusi musanayese.

1. Ikani pambali ma reagents ndi zitsanzo ku kutentha kwa chipinda.
2. Tsegulani Portable Immune Analyzer (WIZ-A101), lowetsani mawu achinsinsi a akaunti malinga ndi njira yogwiritsira ntchito chida, ndikulowetsani mawonekedwe ozindikira.
3. Jambulani kachidindo kuti mutsimikizire chinthucho.
4. Chotsani khadi yoyesera mu thumba la zojambulazo.
5. Lowetsani khadi loyesera mu kagawo ka khadi, jambulani kachidindo ka QR, ndi kuzindikira chinthu choyesera.
6. Onjezani 15μL seramu kapena plasma chitsanzo mu yankho, ndipo sakanizani bwino
7. Onjezani kusakaniza kwa 80μL kuti muyese chitsime cha khadi.
8. Dinani batani la "standard test", patatha mphindi 15, chidacho chidzangozindikira khadi loyesera, chikhoza kuwerenga zotsatira kuchokera pazenera zowonetsera chida, ndikulemba / kusindikiza zotsatira zoyesa.
9. Onani malangizo a Portable Immune Analyzer(WIZ-A101).