MĀTĀPONO

Ko te kiriuhi o te taputapu whakamatautau ka pania ki te conjugate o te BSA me te 25-(OH)VD i te rohe whakamatautau me te koati anti rabbit IgG antibody i runga i te rohe whakahaere.Ka pania te papa tohu ki te tohu fluorescence anti 25-(OH)VD antibody me te IgG rapeti i mua.I te wa e whakamatautau ana i te tauira, 25-(OH)VD i roto i te tauira ka whakakotahi me te rewharewha kua tohua he anti 25-(OH)VD antibody, ka hanga i te ranunga aukati.I raro i te mahi a te immunochromatography, ka rere te matatini ki te huarahi o te pepa absorbent, i te wa i pahemo te matatini i te rohe whakamatautau, Ko te tohu rererangi kore utu ka hono ki te 25- (OH) VD i runga i te membrane.Ko te kukū o te 25- (OH) Ko te VD he hononga toraro mo te tohu rewharewha, a ka kitea te kukū o te 25-(OH)VD i roto i te tauira ma te whakamatautau immunoassay fluorescence.

Tikanga Whakamatau:

Tena koa panuihia te pukapuka mahi taputapu me te whakauru mokihi i mua i te whakamatautau.

1. Whakatakotoria nga reagents me nga tauira katoa ki te taha mahana.
2. Whakatuwheratia te Portable Immune Analyzer(WIZ-A101), tomo i te takiuru kupuhipa pūkete kia rite ki te tikanga whakahaere o te taputapu, ka uru ki te atanga kitenga.
3. Matawaihia te tohu tohu hei whakau i te mea whakamatautau.
4. Tangohia te kaari whakamatautau mai i te putea pepa.
5. Whakauruhia te kaari whakamatautau ki roto i te mokamoka kaari, matawai i te waehere QR, ka whakatau i te mea whakamatautau.
6. Tāpirihia he 15μL serum, tauira plasma ranei ki te otinga, ka uru pai
7. Taapirihia te ranunga 80μL hei tauira pai o te kaari.
8. Patohia te paatene "whakamatautau paerewa", i muri i nga meneti 15, ka kitea e te taputapu te kaari whakamatautau, ka taea e ia te panui i nga hua mai i te mata whakaatu o te taputapu, me te tuhi / tuhi i nga hua whakamatautau.
9. Tirohia te tohutohu a Portable Immune Analyzer(WIZ-A101).