TAƘAITACCE

Vitamin D bitamin ne kuma hormone ne na steroid, wanda ya haɗa da VD2 da VD3, wanda tsarinsa yayi kama sosai. Ana canza Vitamin D3 da D2 zuwa hydroxyl bitamin D 25 (gami da bitamin D3 da D2 25). 25-(OH) VD a jikin ɗan adam, tsari mai ƙarfi, yawan maida hankali. 25-(OH) VD yana nuna jimlar adadin bitamin D, da ikon canzawar bitamin D, don haka ana ɗaukar 25-(OH) VD a matsayin mafi kyawun ma'auni don kimanta matakin bitamin D. Kayan Binciken ya dogara ne akan immunochromatography kuma yana iya bayar da sakamako cikin mintuna 15.

KA'IDUN TSARIN

An rufe membrane na na'urar gwajin da haɗin BSA da 25-(OH)VD a yankin gwaji da kuma antibody na IgG na maganin goat akan yankin sarrafawa. Ana shafa faifan alamar da alamar haske ta antibody na 25-(OH)VD da kuma IgG na zomo a gaba. Lokacin gwada samfurin, 25-(OH)VD a cikin samfurin yana haɗuwa da antibody mai alamar haske ta anti 25-(OH)VD, kuma yana samar da cakuda garkuwar jiki. A ƙarƙashin aikin immunochromatography, kwararar hadaddun a cikin alkiblar takardar sha, lokacin da hadaddun ya wuce yankin gwaji, za a haɗa alamar fluorescent kyauta tare da 25-(OH)VD akan membrane. Yawan 25-(OH)VD yana da alaƙa mara kyau ga siginar fluorescent, kuma yawan 25-(OH)VD a cikin samfurin ana iya gano shi ta hanyar gwajin immunoassay na fluorescence.