TAKAITACCEN

Vitamin D shine bitamin kuma shima hormone ne na steroid, galibi ya haɗa da VD2 da VD3, waɗanda tsarin su yayi kama da juna.Ana canza Vitamin D3 da D2 zuwa 25 hydroxyl bitamin D (ciki har da 25-dihydroxyl bitamin D3 da D2).25- (OH) VD a cikin jikin mutum, tsayayyen tsari, babban taro.25- (OH) VD yana nuna jimlar adadin bitamin D, da ikon canzawa na bitamin D, don haka 25- (OH) VD yana dauke da mafi kyawun alama don kimanta matakin bitamin D. Kit ɗin Diagnostic ya dogara ne akan Immunochromatography kuma yana iya ba da sakamako a cikin mintuna 15.

KA'IDAR HANYA

Ana rufe membrane na na'urar gwajin tare da haɗin BSA da 25- (OH) VD akan yankin gwajin da goat anti-zomo IgG antibody akan yankin sarrafawa.Alamar alamar ana lullube ta da alamar fluorescence anti 25-(OH) VD antibody da zomo IgG a gaba.Lokacin gwajin samfurin, 25- (OH) VD a cikin samfurin haɗa tare da mai walƙiya alama anti 25- (OH) VD antibody, da kuma samar da cakuda rigakafi.A karkashin aikin immunochromatography, da hadaddun ya kwarara a cikin shugabanci na absorbent takarda, a lokacin da hadaddun wuce gwajin yankin, The free kyalli alama za a hade tare da 25- (OH) VD a kan membrane.The maida hankali na 25- (OH) VD ba daidai ba ne don siginar haske, kuma ƙaddamar da 25- (OH) VD a cikin samfurin za a iya gano shi ta hanyar gwajin immunoassay fluorescence.