KAUPAPA

Ko te Huaora D he huaora, he homoni steroid ano hoki, ko te nuinga ko te VD2 me te VD3, he tino rite te hanganga.Ka hurihia te Huaora D3 me te D2 ki te 25 hydroxyl vitamin D (tae atu ki te 25-dihydroxyl vitamin D3 me D2).25-(OH) VD i roto i te tinana tangata, struction pūmau, kukū tiketike.Ko te 25-(OH) VD e whakaatu ana i te tapeke o te huaora D , me te kaha o te hurihanga o te huaora D, na ko te 25-(OH)VD ka kiia ko te tohu pai rawa atu mo te arotake i te taumata o te huaora D. Ko te Kete Tohu Whakaata kei runga immunochromatography a ka puta he hua i roto i te 15 meneti.

MĀTĀPONO O TE TIKANGA

Ko te kiriuhi o te taputapu whakamatautau ka pania ki te conjugate o te BSA me te 25-(OH)VD i te rohe whakamatautau me te koati anti rabbit IgG antibody i runga i te rohe whakahaere.Ka pania te papa tohu ki te tohu fluorescence anti 25-(OH)VD antibody me te IgG rapeti i mua.I te wa e whakamatautau ana i te tauira, 25-(OH)VD i roto i te tauira ka whakakotahi me te rewharewha kua tohua he anti 25-(OH)VD antibody, ka hanga i te ranunga aukati.I raro i te mahi a te immunochromatography, ka rere te matatini ki te huarahi o te pepa absorbent, i te wa i pahemo te matatini i te rohe whakamatautau, Ko te tohu rererangi kore utu ka hono ki te 25- (OH) VD i runga i te membrane.Ko te kukū o te 25- (OH) Ko te VD he hononga toraro mo te tohu rewharewha, a ka kitea te kukū o te 25-(OH)VD i roto i te tauira ma te whakamatautau immunoassay fluorescence.