USHWANKATHELO

I-Vitamin D yivithamin kwaye ikwayi-hormone ye-steroid, ingakumbi i-VD2 kunye ne-VD3, umyalelo wayo ufana kakhulu.I-Vitamin D3 kunye ne-D2 iguqulelwe kwi-25 i-hydroxyl i-vitamin D (kuquka i-25-dihydroxyl i-vitamin D3 kunye ne-D2).I-25-(OH) i-VD emzimbeni womntu, umyalelo ozinzileyo, ukugxila okuphezulu.I-25-(OH) i-VD ibonisa inani elipheleleyo levithamini D, kunye nokukwazi ukuguqulwa kwe-vitamin D, ngoko i-25-(OH) VD ithathwa njengesona salathisi sokuvavanya izinga le-vitamin D.I-Diagnostic Kit isekelwe i-immunochromatography kwaye inokunika umphumo kwimizuzu eyi-15.

UMGAQO WENKQUBO

I-membrane yesixhobo sokuvavanya ifakwe kwi-conjugate ye-BSA kunye ne-25- (OH) VD kwindawo yokuvavanya kunye nebhokhwe yokulwa nomvundla we-IgG kwingingqi yokulawula.Iphedi yesiphawuli iqatywe nge-fluorescence mark anti-25-(OH) VD antibody kunye nomvundla IgG kwangaphambili.Xa uvavanyo lwesampulu, 25-(OH) VD kwisampulu dibanisa fluorescence ephawulwe anti 25-(OH) VD antibody, kwaye wenze umxube omzimba.Ngaphantsi kwesenzo se-immunochromatography, ukuhamba okuyinkimbinkimbi kwicala lephepha elifunxayo, xa i-complex idlula ummandla wokuvavanya, I-marker ye-fluorescent yamahhala iya kudibaniswa ne-25-(OH) VD kwi-membrane.Uxinzelelo lwe-25-(OH) I-VD lulungelelwaniso olubi lwesignali ye-fluorescence, kunye noxinaniso lwe-25-(OH) VD kwisampulu lunokubonwa nge-fluorescence immunoassay assay.