HOOLAHA

ʻO ka Vitamin D he huaʻaola a he hormone steroid pū kekahi, ʻo ia hoʻi me VD2 a me VD3, nona ke ʻano like.Hoʻololi ʻia ka Vitamin D3 a me D2 i 25 hydroxyl vitamin D (me 25-dihydroxyl vitamin D3 a me D2).25-(OH) VD i loko o ke kino kanaka, struction paʻa, kiʻekiʻe ka manaʻo.Hōʻike ka 25-(OH) VD i ka nui o ka huaora D, a me ka hiki ke hoʻololi i ka vitamina D, no laila ua manaʻo ʻia ʻo 25-(OH)VD ka hōʻailona maikaʻi loa no ka loiloi ʻana i ke kiʻekiʻe o ka vitamina D. Hoʻokumu ʻia ka Diagnostic Kit ma immunochromatography a hiki ke hāʻawi i kahi hopena i loko o 15 mau minuke.

KUMU O KE HANA

Hoʻopili ʻia ka membrane o ka mea hoʻāʻo me ka conjugate o BSA a me 25-(OH)VD ma ka ʻāpana hoʻāʻo a me ka anti rabbit IgG antibody ma ka ʻāpana hoʻomalu.Hoʻopili ʻia ka pahu marker e ka fluorescence mark anti 25-(OH)VD antibody a me ka rabbit IgG ma mua.I ka hoʻāʻo ʻana i ka laʻana, 25-(OH)VD i loko o ka laʻana e hui pū me ka fluorescence i kaha ʻia i ka anti 25-(OH)VD antibody, a hana i ka hui ʻole.Ma lalo o ka hana o ka immunochromatography, ka paʻakikī kahe i ke kuhikuhi o ka absorbent pepa, i ka wā paʻakikī i hala i ka ho'āʻo 'āina, E hui 'ia ka mea hoailona fluorescent manuahi me 25-(OH)VD ma ka membrane.The concentration of 25-(OH) ʻO VD ka pilina maikaʻi ʻole no ka hōʻailona fluorescence, a hiki ke ʻike ʻia ke ʻano o 25-(OH)VD i ka laʻana e ka fluorescence immunoassay assay.