CHIDULE

Vitamini D ndi vitamini komanso ndi mahomoni a steroid, makamaka VD2 ndi VD3, omwe malangizo awo ndi ofanana kwambiri.Vitamini D3 ndi D2 amasinthidwa kukhala 25 hydroxyl vitamini D (kuphatikiza 25-dihydroxyl vitamini D3 ndi D2).25-(OH) VD m'thupi la munthu, malangizo okhazikika, okwera kwambiri.25-(OH) VD imasonyeza kuchuluka kwa vitamini D, ndi mphamvu ya kutembenuka kwa vitamini D, kotero 25-(OH) VD imatengedwa kuti ndi chizindikiro chabwino kwambiri chowunika mlingo wa vitamini D. The Diagnostic Kit imachokera immunochromatography ndipo amatha kupereka zotsatira mkati mwa mphindi 15.

MFUNDO YA NJIRA

Nembanemba ya chipangizo choyesera imakutidwa ndi conjugate ya BSA ndi 25-(OH) VD pagawo loyeserera ndi anti-anti rabbit IgG antibody pagawo lowongolera.Marker pad amakutidwa ndi fluorescence mark anti 25-(OH) VD antibody ndi kalulu IgG pasadakhale.Mukayesa zitsanzo, 25-(OH) VD mu zitsanzo phatikizani ndi fluorescence zolembedwa anti 25-(OH)VD antibody, ndi kupanga osakaniza chitetezo.Pansi pa zochita za immunochromatography, kuyenda movutikira kunjira ya pepala loyamwa, pamene zovuta zidadutsa gawo loyesa, Chizindikiro cha fulorosenti yaulere chidzaphatikizidwa ndi 25-(OH) VD pa nembanemba.Kuphatikizika kwa 25-(OH) VD ndikulumikizana koyipa kwa siginecha ya fluorescence, ndipo kuchuluka kwa 25-(OH) VD mu zitsanzo kumatha kuzindikirika ndi fluorescence immunoassay assay.