KA'IDA

Wannan tsiri yana da murfin hana cal McAb a yankin gwaji da kuma maganin rigakafi na IgG na akuya a yankin sarrafawa, wanda aka manne shi da membrane chromatography a gaba. Ana shafa murfin da aka yi da colloidal zinariya mai lakabin anti cal McAb da kuma maganin rigakafi na colloidal zinariya mai lakabin IgG a gaba. Lokacin gwada samfurin mai kyau, samfurin cal a cikin samfurin ya haɗu da colloidal zinariya mai lakabin anti cal McAb, kuma ya samar da hadaddun garkuwar jiki, saboda an ba shi damar yin ƙaura tare da tsiri gwajin, haɗin gwiwar cal conjugate yana kamawa ta hanyar murfin anti cal McAb akan membrane kuma ya samar da hadaddun "mai hana cal McAb-cal-colloidal zinariya mai lakabin anti cal McAb", ƙungiyar gwaji mai launi ta bayyana a yankin gwaji. Ƙarfin launi yana da alaƙa mai kyau da abun ciki na cal. Samfurin mara kyau ba ya samar da ƙungiyar gwaji saboda rashin haɗin cal colloidal zinariya mai haɗawa. Ko da cal yana cikin samfurin ko a'a, akwai layin ja a yankin tunani da yankin kula da inganci, wanda ake ɗauka a matsayin ƙa'idodin kasuwanci na ciki.

Tsarin Gwaji:

1. A cire sandar samfurin, a saka a cikin samfurin najasa, sannan a mayar da sandar samfurin, a matse sosai sannan a girgiza sosai, a maimaita aikin sau 3. Ko kuma a yi amfani da sandar samfurin a ɗauki kimanin 50mg na samfurin najasa, sannan a saka a cikin bututun samfurin najasa wanda ke ɗauke da najasa, sannan a matse sosai.

2. Yi amfani da samfurin pipette da za a iya zubarwa, ɗauki samfurin najasa mai siriri daga mai gudawa, sannan a ƙara digo 3 (kimanin 100uL) a cikin bututun gwajin najasa sannan a girgiza sosai, a ajiye a gefe.

3. Cire katin gwaji daga jakar foil, sanya shi a kan teburin matakin sannan ka yi masa alama.

4. Cire murfin daga bututun samfurin kuma jefar da digo biyu na farko da aka narkar da samfurin, ƙara digo 4. (kimanin 100uL) ba tare da kumfa ba a tsaye kuma a hankali a cikin rijiyar samfurin na katin tare da dispette da aka bayar, fara lokacin.

5. Ya kamata a karanta sakamakon cikin mintuna 10-15, kuma ba shi da inganci bayan mintuna 15.