HŌʻULUʻULU MANAʻO

He huaora ka Vitamin D a he hormone steroid hoʻi, ʻo ia hoʻi ʻo VD2 a me VD3, nona ke ʻano hana like loa. Hoʻololi ʻia ka Vitamin D3 a me D2 i 25 hydroxyl vitamin D (me 25-dihydroxyl vitamin D3 a me D2). 25-(OH) VD i loko o ke kino kanaka, paʻa ke ʻano hana, kiʻekiʻe ka nui. Hōʻike ka 25-(OH) VD i ka nui o ka huaora D, a me ka hiki ke hoʻololi i ka huaora D, no laila ua manaʻo ʻia ʻo 25-(OH) VD ka hōʻailona maikaʻi loa no ka loiloi ʻana i ka pae o ka huaora D. Hoʻokumu ʻia ka Diagnostic Kit ma ka immunochromatography a hiki ke hāʻawi i kahi hopena i loko o 15 mau minuke.

KUMUHANA O KE KAʻINA HANA

Ua uhi ʻia ka membrane o ka mea hoʻāʻo me ka conjugate o BSA a me 25-(OH)VD ma ka ʻāpana hoʻāʻo a me ka antibody IgG kao anti rabbit ma ka ʻāpana kaohi. Ua uhi ʻia ka pad marker e ka fluorescence mark anti 25-(OH)VD antibody a me ka rabbit IgG ma mua. I ka hoʻāʻo ʻana i ka laʻana, hui pū ka 25-(OH)VD i loko o ka laʻana me ka fluorescence i hōʻailona ʻia anti 25-(OH)VD antibody, a hana i ka hui pale. Ma lalo o ka hana o ka immunochromatography, ke kahe nei ka paʻakikī i ke kuhikuhi o ka pepa absorbent, i ka wā i hala ai ka paʻakikī i ka ʻāpana hoʻāʻo, e hui pū ʻia ka marker fluorescent manuahi me 25-(OH)VD ma ka membrane. ʻO ka nui o 25-(OH)VD he pilina maikaʻi ʻole no ka hōʻailona fluorescence, a hiki ke ʻike ʻia ka nui o 25-(OH)VD i loko o ka laʻana e ka fluorescence immunoassay assay.