Ngwa nchọpụta maka Procalcitonin (Nnwale Immnuochromatographic nke Fluorescence)
Ngwa Nchọpụta maka Procalcitonin
(nnwale immunochromatographic fluorescence)
Maka ojiji nchọpụta n'ime vitro naanị
Biko gụọ ihe e tinyere na ngwugwu a nke ọma tupu e jiri ya, ma gbasoo ntuziaka ndị a nke ọma. A gaghị ekwe nkwa na nsonaazụ nnwale ga-adabere na ntụkwasị obi ma ọ bụrụ na e nwere ihe ọ bụla na-adịghị mma na ntuziaka dị na ngwugwu a.
Ebumnobi Ojiji
Ngwa Nchọpụta maka Procalcitonin (nnwale fluorescence immunochromatographic) bụ nnwale fluorescence immunochromatographic maka nchọpụta ọnụọgụ nke Procalcitonin (PCT) na serum ma ọ bụ plasma mmadụ, a na-eji ya maka nchọpụta enyemaka nke ọrịa nje bacteria na sepsis. A ga-eji usoro ndị ọzọ kwado ihe nlele niile dị mma. Emebere ule a maka ojiji ndị ọkachamara ahụike naanị.
Nchịkọta
Procalcitonin nwere amino acid 116, ibu ya dịkwa na molekul bụ 12.7KD. Mkpụrụ ndụ neuroendocrine na-egosipụta PCT ma na-agbaji site na enzymes ka ọ bụrụ calcitonin (etozubeghị oke), peptide carboxy-terminating, na peptide amino terminating. Ndị nwere ahụike nwere obere PCT n'ọbara ha, nke nwere ike ịba ụba nke ukwuu mgbe nje bacteria butere ọrịa. Mgbe sepsis mere n'ahụ, ọtụtụ anụ ahụ nwere ike igosipụta PCT, yabụ enwere ike iji PCT mee ihe dị ka ihe ngosi nke sepsis. Maka ụfọdụ ndị ọrịa nwere ọrịa mbufụt, enwere ike iji PCT mee ihe dị ka ihe ngosi nke nhọrọ ọgwụ nje na mkpebi arụmọrụ.
ỤKPỤRỤ NKE ỤZỌ A
A na-ekpuchi akpụkpọ ahụ nke ngwaọrụ nnwale ahụ na mgbochi nje PCT na mpaghara nnwale ahụ na mgbochi nje IgG nke ewu na mpaghara njikwa. A na-ekpuchi paadị label ahụ site na iji fluorescence nke ejiri akara nje PCT na oke bekee mee ihe na IgG tupu oge eruo. Mgbe a na-anwale ihe nlele dị mma, antigen PCT dị na ihe nlele ahụ na-ejikọta ya na mgbochi nje PCT nke ejiri akara fluorescence mee, ma mepụta ngwakọta mgbochi nje. N'okpuru ọrụ nke immunochromatography, usoro mgbagwoju anya na-aga n'ihu nke akwụkwọ na-amịkọrọ mmiri, mgbe mgbagwoju anya gafere mpaghara nnwale ahụ, ọ jikọtara ya na mgbochi nje PCT nke ejiri akara, na-emepụta mgbagwoju anya ọhụrụ. Ọkwa PCT na-ejikọta nke ọma na mgbaàmà fluorescence, a pụkwara ịchọpụta ntinye nke PCT na ihe nlele ahụ site na nnwale immunoassay fluorescence.
