Ngwa nyocha makaprotein C-reactive hypersensitive

(immunochromatographic nyocha nke fluorescence)

N'ihi na in vitro diagnostic ojiji naanị

Biko gụọ ihe ngwungwu a nke ọma tupu ejiri ya wee soro ntuziaka ahụ.Enweghị ike ịkwenye ntụkwasị obi nke nsonaazụ nyocha ma ọ bụrụ na ọ dị iche na ntuziaka dị na ntinye ngwugwu a.

Ezubere iji

Kit diagnostic maka hypersensitive C-reactive protein (fluorescence immunochromatographic assay) bụ fluorescence immunochromatographic assay maka nyocha quantitative nke C-reactive protein (CRP) na ọbara mmadụ /plasma/ dum ọbara.Ọ bụ ihe na-egosi mbufụt nke na-abụghị nke akọwapụtara.A ga-akwadorịrị nlele niile dị mma site na usoro ndị ọzọ.Ezubere ule a maka naanị ndị ọkachamara ahụike.

Nchịkọta

Protein C-reactive bụ nnukwu protein nke na-emepe emepe site na mkpali lymphokine nke imeju na sel epithelial.Ọ dị na ọbara mmadụ, mmiri ụbụrụ cerebrospinal, pleural na abdominal fluid, wdg, ma bụrụ akụkụ nke usoro nchebe na-abụghị nke a kapịrị ọnụ.6-8h mgbe ihe omume nke nje nje, CRP malitere ịba ụba, 24-48h ruru elu, na ọnụ ọgụgụ kasị elu nwere ike iru ọtụtụ narị ugboro nkịtị.Mgbe e kpochapụrụ ọrịa ahụ, CRP dara nke ọma wee laghachi na nkịtị n'ime otu izu.Otú ọ dị, CRP adịghị amụba nke ọma n'ihe gbasara ọrịa nje, nke na-enye ihe ndabere maka njirimara nke ụdị ọrịa ọrịa n'oge, ma bụrụ ngwá ọrụ iji chọpụta ọrịa nje ma ọ bụ nje.

Ụkpụrụ nke usoro

A na-ekpuchi akpụkpọ ahụ nke ngwaọrụ nnwale ahụ na mgbochi CRP na mpaghara ule yana mgbochi ewu mgbochi oke bekee IgG na mpaghara njikwa.A na-ekpuchi pad lable site na fluorescence akpọrọ mgbochi CRP antibody na oke bekee IgG tupu oge eruo.Mgbe a na-anwale nlele dị mma, antigen CRP dị na sample jikọtara ya na fluorescence akpọrọ mgbochi CRP antibody, wee mepụta ngwakọta mgbochi.N'okpuru omume nke immunochromatography, mgbagwoju eruba na ntụziaka nke absorbent akwụkwọ, mgbe mgbagwoju gafere ule mpaghara, ọ jikọtara na mgbochi CRP mkpuchi mgbochi, na-etolite ọhụrụ mgbagwoju.A na-ejikọta ọkwa CRP nke ọma na mgbaàmà fluorescence, na ntinye nke CRP na sample nwere ike chọpụta site na fluorescence immunoassay assay.

Reagent na ihe ndị e nyere

Ngwa ngwugwu 25T:

Kaadị ule n'otu n'otu ejiri akpa 25T desiccant tinye ya

Ihe atụ diluents 25T

Ntinye ngwugwu 1

AKWỤKWỌ KWESỊRỊ MA Ọ BỤGHỊ

Akpa mkpokọta ihe nlele, ngụ oge

NKWUKWU NA NKWUKWU

1. Ihe nlele a nwalere nwere ike ịbụ serum, heparin anticoagulant plasma ma ọ bụ EDTA anticoagulant plasma.
2. Dị ka ọkọlọtọ usoro na-anakọta sample.Enwere ike idowe ihe nlele ọbara ma ọ bụ plasma refrigerated na 2-8 ℃ maka ụbọchị 7 na cryopreservation n'okpuru -15 Celsius maka ọnwa 6.Enwere ike idowe ihe nlele ọbara niile na friji na 2-8 ℃ maka ụbọchị 3
3. Nlereanya niile na-ezere okirikiri ịsa oyi.