Ngwa Nchọpụta makaprotein C-reactive hypersensitive

(nnwale immunochromatographic fluorescence)

Maka ojiji nchọpụta n'ime vitro naanị

Biko gụọ ihe e tinyere na ngwugwu a nke ọma tupu e jiri ya, ma gbasoo ntuziaka ndị a nke ọma. A gaghị ekwe nkwa na nsonaazụ nnwale ga-adabere na ntụkwasị obi ma ọ bụrụ na e nwere ihe ọ bụla na-adịghị mma na ntuziaka dị na ngwugwu a.

Ebumnobi Ojiji

Ngwa nchọpụta maka protein C-reactive hypersensitive (nnwale fluorescence immunochromatographic) bụ nnwale fluorescence immunochromatographic maka nchọpụta ọnụọgụ nke protein C-reactive (CRP) na serum /plasma / ọbara mmadụ dum. Ọ bụ ihe na-egosi mbufụt na-abụghị nke a kapịrị ọnụ. A ga-eji usoro ndị ọzọ kwado ihe nlele niile dị mma. Emebere ule a maka ojiji ndị ọkachamara ahụike naanị.

Nchịkọta

Protein C-reactive bụ protein nke a na-akpọ "acute phase protein" nke na-eme ka mkpụrụ ndụ imeju na epithelial nwee ike ịrụ ọrụ. Ọ dị na serum mmadụ, mmiri cerebrospinal, mmiri pleural na afọ, wdg, ọ bụkwa akụkụ nke usoro ahụ ji alụso ọrịa ọgụ na-abụghị nke a kapịrị ọnụ. Awa 6-8 ka ọrịa nje bacteria malitere, CRP malitere ịba ụba, awa 24-48 ruru oke, ọnụ ọgụgụ kachasị elu nwere ike iru ọtụtụ narị ugboro nke nkịtị. Mgbe ewepụsịrị ọrịa ahụ, CRP dara nke ukwuu ma laghachi na nkịtị n'ime otu izu. Agbanyeghị, CRP anaghị abawanye nke ukwuu n'ihe gbasara ọrịa nje, nke na-enye ntọala maka ịchọpụta ụdị ọrịa mbụ, ọ bụkwa ngwaọrụ maka ịchọpụta ọrịa nje ma ọ bụ nje bacteria.

ỤKPỤRỤ NKE ỤZỌ A

A na-ekpuchi akpụkpọ ahụ nke ngwaọrụ nnwale ahụ na mgbochi CRP na mpaghara nnwale ahụ na mgbochi IgG nke ewu na mpaghara njikwa. A na-ekpuchi paadị label ahụ site na iji fluorescence nke ejiri akara anti CRP na oke bekee mee ihe tupu oge eruo. Mgbe a na-anwale ihe nlele dị mma, antigen CRP dị na ihe nlele ahụ na-ejikọta ya na mgbochi CRP nke ejiri akara fluorescence mee, ma mepụta ngwakọta mgbochi. N'okpuru ọrụ nke immunochromatography, usoro mgbagwoju anya na-aga n'ihu nke akwụkwọ na-amịkọrọ mmiri, mgbe mgbagwoju anya gafere mpaghara nnwale ahụ, ọ jikọtara ya na mgbochi CRP nke ejiri akara antibody kpuchie, na-emepụta mgbagwoju anya ọhụrụ. Ọkwa CRP na-ejikọta nke ọma na mgbaàmà fluorescence, a pụkwara ịchọpụta ntinye nke CRP na ihe nlele ahụ site na nnwale immunoassay fluorescence.

Ihe ndị na-eme ka ihe dị nro na ihe ndị e ji mee ihe dị iche iche

Ihe mejupụtara ngwugwu 25T:

Kaadị nnwale n'otu n'otu nke ejiri foil kpuchie akpa ya na ihe nhicha mmiri 25T

Ihe ndị na-agbaze ihe nlele 25T

Ntinye ngwugwu 1

A CHỌRỌ IHE E JI MEE YA MA E NWEGHỊ YA

Ihe nlele nchịkọta akpa, ihe ngụ oge

Nchịkọta na Nchekwa ihe nlele

1. Ihe nlele ndị a nwalere nwere ike ịbụ serum, heparin anticoagulant plasma ma ọ bụ EDTA anticoagulant plasma.
2. Dịka usoro ọkọlọtọ si dị, a pụrụ iji ihe nlele ọbara maọbụ plasma tinye ya n'ime friji na okpomọkụ 2-8℃ ruo ụbọchị asaa, ebe a na-echekwa ya n'okpuru -15°C ruo ọnwa isii. A pụrụ idobe ihe nlele ọbara dum n'ime friji na okpomọkụ 2-8℃ ruo ụbọchị atọ.
3. Ihe nlele niile na-ezere usoro ịgbaze na ịgbaze.