Nchịkọta

Vitamin D bụ vitamin ma bụrụkwa homonụ steroid, nke gụnyere VD2 na VD3, nke usoro ha yiri nnọọ. Vitamin D3 na D2 na-atụgharị gaa na 25 hydroxyl vitamin D (gụnyere 25-dihydroxyl vitamin D3 na D2). 25- (OH) VD n'ime ahụ mmadụ, nkwụsi ike struction, elu itinye uche. 25- (OH) VD na-egosipụta ọnụọgụ vitamin D zuru oke, na ikike ngbanwe nke vitamin D, ya mere 25- (OH) VD na-ewere dị ka ihe ngosi kachasị mma maka ịlele ọkwa vitamin D. Ihe nchọpụta nchọpụta dabeere na immunochromatography ma nwee ike inye nsonaazụ n'ime 15 nkeji.

Ụkpụrụ nke usoro

A na-ekpuchi akpụkpọ ahụ nke ngwaọrụ ule na conjugate nke BSA na 25- (OH) VD na mpaghara ule na ewu mgbochi oke bekee IgG na mpaghara nchịkwa. A na-ekpuchi pad ihe nrịbama site na akara fluorescence anti 25-(OH) VD mgbochi na oke bekee IgG tupu oge eruo. Mgbe a na-anwale sample, 25- (OH) VD na sample jikọtara na fluorescence akara mgbochi 25- (OH) VD mgbochi, na-etolite dịghịzi ngwakọta. N'okpuru omume nke immunochromatography, mgbagwoju eruba na ntụziaka nke absorbent akwụkwọ, mgbe mgbagwoju gafere ule mpaghara, The free fluorescent marker ga-ejikọta ya na 25- (OH) VD na membrane. The ịta nke 25- (OH) VD bụ adịghị mma mmekọrịta maka fluorescence mgbaàmà, na ịta nke 25- (OH) VD na sample nwere ike chọpụta site flu.