Nchịkọta

Vitamin D bụ vitamin ma bụrụkwa hormone steroid, nke gụnyere VD2 na VD3, nke nhazi ya yiri nke ukwuu. A na-agbanwe Vitamin D3 na D2 ka ọ bụrụ vitamin D hydroxyl 25 (gụnyere vitamin D3 na D2 25). 25-(OH) VD n'ahụ mmadụ, nhazi siri ike, oke njupụta. 25-(OH) VD na-egosipụta mkpokọta vitamin D, yana ikike mgbanwe nke vitamin D, yabụ a na-ewere 25-(OH) VD dị ka ihe ngosi kacha mma maka ịtụle ọkwa vitamin D. Ngwa nchọpụta ahụ dabere na immunochromatography ma nwee ike inye nsonaazụ n'ime nkeji iri na ise.

ỤKPỤRỤ NKE ỤZỌ A

A na-ekpuchi akpụkpọ ahụ nke ngwaọrụ nnwale ahụ na njikọ nke BSA na 25-(OH)VD na mpaghara nnwale ahụ na antibody IgG anti rabbit na mpaghara njikwa. A na-ekpuchi mpempe akara ahụ site na ihe mgbochi fluorescence mark anti 25-(OH)VD na rabbit IgG tupu oge eruo. Mgbe a na-anwale ihe nlele ahụ, 25-(OH)VD na ihe nlele ahụ na-ejikọta ya na antibody anti 25-(OH)VD akara fluorescence, wee mepụta ngwakọta mgbochi. N'okpuru ọrụ nke immunochromatography, usoro mgbagwoju anya na-aga n'ihu nke akwụkwọ na-amịkọrọ mmiri, mgbe mgbagwoju anya gafere mpaghara nnwale ahụ, a ga-ejikọta akara fluorescence efu na 25-(OH)VD na membrane ahụ. Ntinye nke 25-(OH)VD bụ njikọ na-adịghị mma maka mgbaàmà fluorescence, enwere ike ịchọpụta ntinye nke 25-(OH)VD na ihe nlele ahụ site na nnwale immunoassay fluorescence.