TE MATUA

Kua pania te kiriuhi o te taputapu whakamātautau ki te paturopi Cpn-IgM i te rohe whakamātautau, me te paturopi IgG rāpeti koati i te rohe whakahaere. Kua pania te papa tapanga ki te paturopi anti-Cpn-IgM me te IgG rāpeti kua tohua i mua. I te whakamātautau i te tauira pai, ka honoa te antigen Cpn-IgM i roto i te tauira ki te paturopi anti-Cpn-IgM kua tohua i te tohu fluorescence, ā, ka hanga he ranunga ārai mate. I raro i te mahi a te immunochromatography, ka rere te matatini i te ahunga o te pepa mimiti. Ina paahitia e te matatini te rohe whakamātautau, ka honoa ki te paturopi paninga anti-Cpn-IgM, ka hanga he matatini hou. Ko te rārangi whero te paerewa e puta ana i te rohe whakahaere kounga (C) hei whakatau mena he nui ngā tauira, me te mea he noa te tukanga chromatography. Ka whakamahia hoki hei paerewa whakahaere ā-roto mō ngā matū.

Tukanga Whakamātautau

E pā ana te tukanga whakamātautau WIZ-A202 me te taputapu tātari ārai mate kawe WIZ-A101 ki ngā tohutohu o te tohutohu. Ko te tukanga whakamātautau tirohanga koia tēnei:

1. Tangohia te kāri whakamātautau mai i te putea pepa konutea, whakatakotoria ki runga i te tēpu papatahi, ka tohua.
2. Tāpirihia he 10μl o te tauira toto, toto toto rānei, 20ul rānei o te tauira toto katoa ki te puna tauira o te kāri me te dispette kua whakaratohia, kātahi ka tāpirihia he 100μl (tata ki te 2-3 pata) o te wai whakamātao tauira; tīmatahia te wā.
3. Taria mō te iti rawa 10-15 meneti, kātahi ka pānui i te hua, kāore e whai mana te hua i muri i te 15 meneti.