Kete Tātaritanga mō te Procalcitonin

(whakamātautau ārai-ira-whakaahua-whakaahua)

Mō te whakamahinga tātaritanga in vitro anake

Pānuihia tēnei tāpiringa i mua i te whakamahinga, ā, me whai pūmau i ngā tohutohu. Kāore e taea te whakamana i te pono o ngā hua whakamātautau mēnā he rerekētanga mai i ngā tohutohu kei roto i tēnei tāpiringa.

TE WHAKAMAHINGA I WHAKATAURANGIA

Ko te Kete Tātaritanga mō te Procalcitonin (whakamātautau immunochromatographic fluorescence) he whakamātautau immunochromatographic fluorescence mō te kimi i te Procalcitonin (PCT) i roto i te toto tangata, i te plasma rānei, e whakamahia ana mō te tātaritanga āwhina o te mate kitakita me te sepsis. Me whakaū te tauira pai katoa mā ētahi atu tikanga. Ko tēnei whakamātautau he mea hanga mō te whakamahinga hauora anake.

WHAKARĀPOPOTONGA

He 116 ngā waikawa amino kei roto i te procalcitonin, ā, ko tōna taumaha ngota he 12.7KD. Ka whakaatuhia te PCT e ngā pūtau neuroendocrine, ā, ka pakaruhia e ngā whākōkī ki te calcitonin (koretake), te peptide carboxy-terminating, me te peptide amino terminating. He iti noa te PCT i roto i te toto o te hunga hauora, ka taea te whakanui ake i muri i te pangia e te huakita. Ina puta te sepsis i roto i te tinana, ka taea e te nuinga o ngā kiko te whakaatu i te PCT, nō reira ka taea te whakamahi i te PCT hei tohu matapae mō te sepsis. Mō ētahi tūroro e pangia ana e te mate mumura, ka taea te whakamahi i te PCT hei tohu mō te kōwhiringa paturopi me te whakatau whai huatanga.

TE MĀTĀPONO O TE TIKANGA

Kua pania te kiriuhi o te taputapu whakamātautau ki te paturopi anti-PCT i te rohe whakamātautau, me te paturopi anti-rāpeti koati IgG i te rohe whakahaere. Kua pania te papa tapanga ki te paturopi anti-PCT me te IgG rāpeti kua tohua ki te fluorescence i mua i te whakamātautau. I te whakamātautau i te tauira pai, ka honoa te antigen PCT i roto i te tauira ki te paturopi anti-PCT kua tohua ki te fluorescence, ka hanga he ranunga ārai mate. I raro i te mahi a te immunochromatography, ka rere te matatini i te ahunga o te pepa mimiti, ina paahitia e te matatini te rohe whakamātautau, ka honoa ki te paturopi paninga anti-PCT, ka hanga he matatini hou. He hononga pai te taumata PCT ki te tohu fluorescence, ā, ka taea te kite i te kukū o te PCT i roto i te tauira mā te whakamātautau immunoassay fluorescence.