Kete Tātaritanga（RATEKI）mō te Rotavirus Rōpū A
Mō te whakamahinga tātaritanga in vitro anake
Pānuihia tēnei tāpiringa i mua i te whakamahinga, ā, me whai pūmau i ngā tohutohu. Kāore e taea te whakamana i te pono o ngā hua whakamātautau mēnā he rerekētanga mai i ngā tohutohu kei roto i tēnei tāpiringa.

TE WHAKAMAHINGA I WHAKATAURANGIA
He pai te Kete Tātaritanga (LATEX) mō te Rotavirus Rōpū A mō te kimi kounga o te antigen Rotavirus Rōpū A i roto i ngā tauira paru tangata. Mō ngā tohunga hauora anake tēnei whakamātautau. I taua wā, ka whakamahia tēnei whakamātautau mō te tātaritanga haumanu o te mate korere pēpi i roto i ngā tūroro e pangia ana e te mate Rotavirus Rōpū A.

RAHI O TE MŌKIHI
1 kete/pouaka, 10 kete/pouaka, 25 kete,/pouaka, 50 kete/pouaka.

WHAKARĀPOPOTONGA
Kua whakarōpūtia te rotavirus heihuaketo rotate momo o te huaketo exenteral, he āhua porowhita tōna, ā, he 70nm te whānui. Kei roto i te rotavirus he 11 ngā wāhanga o te RNA aho-rua. Ka taea te wehewehe i te rotavirus ki ngā rōpū e whitu (ag) i runga i ngā rerekētanga antigenic me ngā āhuatanga ira. Kua pūrongohia ngā mate tangata o te rōpū A, rōpū B me te rōpū C rotavirus. Ko te Rotavirus Rōpū A te tino take o te gastroenteritis kino i roto i ngā tamariki puta noa i te ao.^[1-2].

TE TIKANGA WHAKAMĀTAUTAU
1. Tangohia te rākau tauira kua whakaurua ki roto i te tauira paru, kātahi ka whakahokia te rākau tauira, ka whakapiripiri kia mau, ka rūrū kia pai, ka whakahokia te mahi kia 3 ngā wā. Mā te whakamahi rānei i te rākau tauira, ka kōwhiria te tauira paru kia 50mg pea, ka whakauru ki roto i te ngongo tauira paru kei roto te waimeha tauira, ka whakapiripiri kia mau.
2. Whakamahia he pipette tango tauira, tangohia te tauira paru angiangi mai i te tūroro mate korere, kātahi ka tāpirihia kia 3 ngā pata (tata ki te 100uL) ki te ngongo tauira paru, ka rūrū pai, ka waiho ki tahaki.
3. Tangohia te kāri whakamātautau mai i te putea pepa konutea, whakatakotoria ki runga i te tēpu papatahi, ka tohua.
4. Tangohia te taupoki i te ngongo tauira ka maka atu ngā pata tuatahi e rua o te tauira kua whakaranua, tāpirihia kia 3 ngā pata (tata ki te 100µL) o te tauira kua whakaranua kia poutū, ka ata ringihia ki roto i te poka tauira o te kāri me te putanga kua whakaratohia, tīmatahia te wā.
5. Me pānui te hua i roto i ngā meneti 10-15, ā, kāore e whai mana i muri i ngā meneti 15.