TE MATUA

Kua pania te kiriuhi o te taputapu whakamātautau ki te paturopi FOB i te rohe whakamātautau, ā, ko te paturopi IgG anti-rāpeti koati i te rohe whakahaere. Kua pania te papa tapanga ki te paturopi anti-FOB me te IgG rāpeti kua tohua ki te fluorescence. I te whakamātautau i te tauira pai, ka honoa te antigen FOB i roto i te tauira ki te paturopi anti-FOB kua tohua ki te fluorescence, ā, ka hanga he ranunga ārai mate. I raro i te mahi a te immunochromatography, ka rere te matatini i te ahunga o te pepa mimiti. Ina paahitia e te matatini te rohe whakamātautau, ka honoa ki te paturopi paninga anti-FOB, ka hanga he matatini hou. Mena he kino, kāore he hemoglobin tangata i roto i te tauira, he iti rānei te ihirangi, kāore e taea te hanga i ngā matatini ārai mate e ranea ana. Kāore he rārangi whero i te rohe kitenga (T). Ko te rārangi whero te paerewa e puta ana i te rohe whakahaere kounga (C) hei whakatau mena he nui ngā tauira, me te mea he noa te tukanga chromatography. Ka whakamahia hoki hei paerewa whakahaere ā-roto mō ngā matū.

Tukanga Whakamātautau:

Pānuihia te tāpiringa ki te kete i mua i te whakamātautau.

1. Tangohia te kāri whakamātautau mai i te putea pepa konutea, whakatakotoria ki runga i te tēpu papatahi, ka tohua.

2. Tangohia te taupoki i te ngongo tauira, ka maka atu ngā pata tuatahi e rua o te tauira kua whakaranua, tāpirihia kia 3 ngā pata (tata ki te 100µL) o te tauira kua whakaranua, kia poutū, kia āta haere ki roto i te poka tauira o te kāri me te dispette kua whakaratohia. Kātahi ka tīmata te taima.

3. Me pānui te hua i roto i ngā meneti 10-15, ā, kāore e whai mana i muri i ngā meneti 15.