CHIDULE

Vitamini D ndi vitamini ndipo ndi hormone ya steroid, makamaka kuphatikiza VD2 ndi VD3, yomwe kapangidwe kake kamafanana kwambiri. Vitamini D3 ndi D2 amasinthidwa kukhala 25 hydroxyl vitamini D (kuphatikiza 25-dihydroxyl vitamini D3 ndi D2). 25-(OH) VD m'thupi la munthu, kapangidwe kokhazikika, kuchuluka kwakukulu. 25-(OH) VD imasonyeza kuchuluka kwa vitamini D, komanso mphamvu ya kusintha kwa vitamini D, kotero 25-(OH)VD imaonedwa kuti ndi chizindikiro chabwino kwambiri chowunikira kuchuluka kwa vitamini D. Diagnostic Kit imachokera pa immunochromatography ndipo ingapereke zotsatira mkati mwa mphindi 15.

MFUNDO YA NJIRAYI

Nembanemba ya chipangizo choyesera imakutidwa ndi conjugate ya BSA ndi 25-(OH)VD pamalo oyesera ndi antibody ya mbuzi ya IgG pamalo olamulira. Mapepala olembera amakutidwa ndi antibody ya fluorescence mark anti 25-(OH)VD ndi antibody ya kalulu IgG pasadakhale. Poyesa chitsanzo, 25-(OH)VD mu chitsanzo imaphatikizana ndi antibody ya fluorescence mark antibody ya 25-(OH)VD, ndikupanga chisakanizo cha chitetezo chamthupi. Pogwiritsa ntchito immunochromatography, kuyenda kwa zovuta kumayang'ana pepala loyamwa, pamene zovutazo zadutsa pamalo oyesera, chizindikiro cha fluorescent chaulere chidzaphatikizidwa ndi 25-(OH)VD pa nembanemba. Kuchuluka kwa 25-(OH)VD ndi mgwirizano woipa wa chizindikiro cha fluorescence, ndipo kuchuluka kwa 25-(OH)VD mu chitsanzo kumatha kuzindikirika ndi fluorescence immunoassay assay.