MFUNDO

Chingwecho chili ndi anti-cal coating McAb pamalo oyesera ndi anti-rabbit IgG antibody ya mbuzi pamalo olamulira, yomwe imalumikizidwa ku membrane chromatography pasadakhale. Lable pad imakutidwa ndi colloidal gold yolembedwa anti-cal McAb ndi colloidal gold yolembedwa anti-IgG antibody ya kalulu pasadakhale. Poyesa chitsanzo chabwino, cal mu chitsanzocho imaphatikizidwa ndi colloidal gold yolembedwa anti-cal McAb, ndipo imapanga immune complex, chifukwa imaloledwa kusuntha motsatira mzere woyesera, cal conjugate complex imagwidwa ndi anti-cal coating McAb pa nembanemba ndikupanga "anti-cal coating McAb-cal-colloidal gold yolembedwa anti-cal McAb", gulu loyesera lamitundu limawonekera pamalo oyesera. Mphamvu ya utoto imagwirizana bwino ndi kuchuluka kwa cal. Chitsanzo choyipa sichipanga gulu loyesera chifukwa chosakhalapo colloidal gold conjugate cal complex. Kaya cal ilipo mu chitsanzo kapena ayi, pali mzere wofiira womwe umawonekera pachigawo chofotokozera ndi dera lowongolera khalidwe, lomwe limawonedwa ngati miyezo yamkati yamakampani abwino.

Njira Yoyesera:

1. Tulutsani ndodo yoyesera, yoyikidwa mu ndowe, kenako ikani ndodo yoyesera kumbuyo, ikani mwamphamvu ndikugwedeza bwino, bwerezani zomwezo katatu. Kapena pogwiritsa ntchito ndodo yoyesera yomwe mwasankha chitsanzo cha ndowe ya 50mg, ndikuyika mu chubu choyesera ndowe chokhala ndi kusungunuka kwa chitsanzo, ndikuyiyika mwamphamvu.

2. Gwiritsani ntchito njira yochotsera ndowe zopyapyala. Tengani chitsanzo cha ndowe zopyapyala kuchokera kwa wodwala matenda otsegula m'mimba, kenako onjezerani madontho atatu (pafupifupi 100uL) mu chubu chochotsera ndowe ndikugwedeza bwino, muyike pambali.

3. Tulutsani khadi loyesera kuchokera mu thumba la foil, liyikeni patebulo lolingana ndikuliyika chizindikiro.

4. Chotsani chivundikirocho mu chubu cha chitsanzo ndikutaya madontho awiri oyamba a chitsanzo chochepetsedwa, onjezani madontho 4 (pafupifupi 100uL) osasungunuka ndi thovu molunjika kenako pang'onopang'ono mu chitsime cha chitsanzo cha khadi ndi dispette yoperekedwa, yambani nthawi.

5. Zotsatira zake ziyenera kuwerengedwa mkati mwa mphindi 10-15, ndipo sizigwira ntchito patatha mphindi 15