Agakoresho ko gusuzuma indwara kagenewePoroteyine ya C-reactive ishobora kwanduza cyane

(isuzuma rya fluorescence immunochromatographic assay)

Gukoreshwa mu gusuzuma indwara mu buryo bwa "in vitro" gusa

Mbere yo gukoresha iyi paki, soma witonze kandi ukurikize amabwiriza neza. Ntabwo bishoboka kwemeza ko ibisubizo by'isuzuma ari ukuri niba hari ibitagenda neza ku mabwiriza ari muri iyi paki.

IKORESHWA RYITEGANYANYE

Ikizamini cyo gusuzuma poroteyine ya C-reactive ifite ubwiyongere bwinshi (fluorescence immunochromatographic assay) ni ikizamini cya fluorescence immunochromatographic assay cyo gupima poroteyine ya C-reactive (CRP) mu maraso y'umuntu /plasma / yose. Ni ikimenyetso kidasobanutse cy'ububyimbirwe. Ikizamini cyose cyabonetse kigomba kwemezwa n'ubundi buryo. Iki kizamini kigenewe gukoreshwa n'abaganga gusa.

INSHAMAKE

Poroteyine ya C-reactive ni poroteyine ikora mu gihe cy’ubuhumekero ikorwa n’uburyo bwo gukangura uturemangingo tw’umwijima n’uturemangingo tw’inyuma. Iboneka mu maraso y’umuntu, mu mazi yo mu bwonko, mu mazi yo mu nda no mu nda, nibindi, kandi ni kimwe mu bigize ubudahangarwa bw’umubiri butari bwihariye. Nyuma y’amasaha 6-8 habayeho ubwandu bwa bagiteri, CRP yatangiye kwiyongera, amasaha 24-48 agera ku rwego rwo hejuru, kandi agaciro kayo gashobora kugera ku nshuro amagana z’ubusanzwe. Nyuma yo gukuraho ubwandu, CRP yaragabanutse cyane isubira ku rwego rwo hejuru mu cyumweru kimwe. Ariko, CRP ntiyongera cyane mu gihe cy’ubwandu bwa virusi, ibyo bikaba ari byo bitanga ishingiro ryo kumenya ubwoko bw’indwara zandura hakiri kare, kandi ni igikoresho cyo kumenya ubwandu bwa virusi cyangwa bagiteri.

IHAME RY'UBURYO BWAYO

Agace k'igikoresho cyo gupima gatwikiriwe na antibody ya CRP ku gace k'igeragezwa naho antibody ya IgG ya antibody y'ihene ku gace k'igenzura. Udupapuro two gupima dutwikiriwe na antibody ya CRP yanditseho fluorescence na IgG ya rabbit mbere y'igihe. Mu gusuzuma icyitegererezo cyagaragaje ko ari cyiza, antigen ya CRP iri mu cyitegererezo ihuzwa na antibody ya CRP yanditseho fluorescence, maze igakora uruvange rw'ubudahangarwa. Mu gihe cy'imikorere ya immunochromatography, urujya n'uruza rw'uruvange mu cyerekezo cy'impapuro zifata, iyo complex yanyuze mu gace k'igeragezwa, yahujwe na antibody ya CRP, ikora complex nshya. Urwego rwa CRP rufitanye isano ryiza n'ikimenyetso cya fluorescence, kandi ingano ya CRP mu cyitegererezo ishobora kumenyekana hakoreshejwe isuzuma rya fluorescence immunoassay.

IBIKORESHO N'IBIKORESHO BYATANZWE

Ibice by'ipaki bya 25T:

Ikarita yo gupimisha ifite agapfunyika k'umuti wo koza amenyo ka 25T

Ingero z'ibinyabutabire 25T

Agasanduku k'ipaki 1

IBIKORESHO BIRAKENEWE ARIKO NTIBYATANGWA

Agasanduku ko gukusanya ingero, igihe

ICYITEGEREREZO CYO GUKORESHA NO KUBIKA

1. Ingero zapimwe zishobora kuba serum, heparin anticoagulant plasma cyangwa EDTA anticoagulant plasma.
2. Dukurikije uburyo busanzwe bwo gukusanya icyitegererezo. Icyitegererezo cya serum cyangwa plasma gishobora kubikwa muri firigo kuri 2-8°C mu gihe cy'iminsi 7 no kubikwa munsi ya -15°C mu gihe cy'amezi 6. Icyitegererezo cy'amaraso yose gishobora kubikwa muri firigo kuri 2-8°C mu gihe cy'iminsi 3.
3. Ingero zose zirinda gukonjesha no gushonga.