INSHAMAKE

Vitamine D ni vitamine kandi ni imisemburo ya steroid, cyane cyane irimo VD2 na VD3, imiterere yayo isa cyane. Vitamine D3 na D2 bihindurwamo 25 hydroxyl vitamine D (harimo 25-dihydroxyl vitamine D3 na D2). 25-(OH) VD mu mubiri w'umuntu, imiterere ihamye, ubwinshi bwayo bwinshi. 25-(OH) VD igaragaza ingano yose ya vitamine D, n'ubushobozi bwa vitamine D mu guhindura, bityo 25-(OH)VD ifatwa nk'ikimenyetso cyiza cyo gusuzuma urwego rwa vitamine D. Iyi Diagnostic Kit ishingiye kuri immunochromatography kandi ishobora gutanga igisubizo mu minota 15.

IHAME RY'UBURYO BWAYO

Agace k'igikoresho cyo gupima gatwikiriwe n'agace ka BSA na 25-(OH)VD ku gace k'igeragezwa n'agace ka anti-inkwavu ka IgG ku gace kagenzurwa. Agapapuro k'ikarita gatwikiriwe n'agace ka anti-inkwavu ka 25-(OH)VD kari ku gace kagenzurwa mbere y'igihe. Mu gihe cyo gupima icyitegererezo, 25-(OH)VD mu gipimo ihuzwa n'agace ka anti-inkwavu ka 25-(OH)VD kari ku gace kagenzurwa, maze bigakora uruvange rw'ubudahangarwa bw'umubiri. Mu gihe cy'igikorwa cya immunochromatography, urujya n'uruza rw'uruvange mu cyerekezo cy'impapuro zifata, iyo agace kanyuze mu gace kagenzurwa, ikimenyetso cya fluorescent cyigenga kizahuzwa na 25-(OH)VD ku gace kagenzurwa. Igipimo cya 25-(OH)VD ni isano iri hagati y'ikimenyetso cya fluorescence, kandi igipimo cya 25-(OH)VD mu gipimo gishobora kumenyekana hakoreshejwe isuzuma ry'ubudahangarwa bw'umubiri.