INCAMAKE

Vitamine D ni vitamine kandi ni na hormone ya steroid, cyane cyane harimo VD2 na VD3, imitekerereze yayo isa cyane. Vitamine D3 na D2 bihindurwamo vitamine D ya hydroxyl 25 (harimo vitamine D-25 ya dihydroxyl D3 na D2). 25- (OH) VD mumubiri wumuntu, guhagarara neza, kwibanda cyane. V- 25-

IHame RY'UBURYO

Ibice by'igikoresho cyo kwipimisha bisizwe hamwe na conjugate ya BSA na 25- (OH) VD mukarere k'ibizamini hamwe na antibody y'ihene irwanya urukwavu IgG mukarere kayobora. Ikimenyetso cya marike gitwikiriwe na fluorescence ikimenyetso kirwanya antibody 25- (OH) VD hamwe ninkwavu IgG mbere. Iyo ugerageza icyitegererezo, 25- (OH) VD muri sample ikomatanya na fluorescence yanditseho antibody 25- (OH) VD, hanyuma igakora imvange yumubiri. Mugihe cyibikorwa bya immunochromatografiya, urujya n'uruza rugana mu cyerekezo cyimpapuro zinjira, mugihe uruganda rwanyuze mukarere kizamini, Ikimenyetso cya fluorescent yubusa kizahuzwa na 25- (OH) VD kuri membrane.Ubushuhe bwa 25- (OH) VD ni ihuriro ribi kubimenyetso bya fluorescence, kandi kwibumbira hamwe kwa fluorescence immunoassay.