PFUPISO

Vhitamini D ivhitamini uye zvakare ihormone yesteroid, kunyanya inosanganisira VD2 neVD3, ine chimiro chakafanana zvikuru. Vhitamini D3 neD2 zvinoshandurwa kuita 25 hydroxyl vitamin D (kusanganisira 25-dihydroxyl vitamin D3 neD2). 25-(OH) VD mumuviri wemunhu, chimiro chakasimba, huwandu hwakawanda. 25-(OH) VD inoratidza huwandu hwese hwevhitamini D, uye kugona kwekushandura kwevhitamini D, saka 25-(OH)VD inoonekwa sechiratidzo chakanakisa chekuongorora huwandu hwevhitamini D. Diagnostic Kit yakavakirwa pa immunochromatography uye inogona kupa mhedzisiro mukati memaminitsi gumi nemashanu.

NZVIMBO YEKUTUNGAMIRIRA

Kamudzira kemudziyo wekuyedza kakaputirwa neBSA ne25-(OH)VD panzvimbo yekuyedza uye antibody yembudzi yeIgG antibody iri panzvimbo yekudzora. Mapedhi emarker anoputirwa neantibody yefluorescence mark anti25-(OH)VD uye antibody yetsuro IgG pachine nguva. Pakuyedza sampuro, 25-(OH)VD mumuenzaniso inosanganiswa neantibody yefluorescence mark anti25-(OH)VD, uye inoumba musanganiswa we immune. Pasi pekushanda kwe immunochromatography, kuyerera kwakaomarara kunoenda kune bepa rinonyudza, kana complex yapfuura nzvimbo yekuyedza, chiratidzo chefree fluorescent chichabatanidzwa ne25-(OH)VD pamembrane. Kuwanda kwe25-(OH)VD inyaya ye negative correlation ye fluorescence signal, uye kuwanda kwe25-(OH)VD mumuenzaniso kunogona kuonekwa ne fluorescence immunoassay assay.