NHEYO

Mutsetse uyu une anti-cal coating McAb panzvimbo yekuyedza uye anti-rabbit IgG antibody yembudzi panzvimbo yekudzora, iyo inosungirirwa ku membrane chromatography pachine nguva. Lable pad inoputirwa ne colloidal gold yakanyorwa anti-cal McAb uye colloidal gold yakanyorwa rabbit IgG antibody pachine nguva. Pakuyedza positive sample, cal iri mu sample inosanganiswa ne colloidal gold yakanyorwa anti-cal McAb, uye inoumba immune complex, sezvo ichibvumirwa kufamba pamwe chete ne test strip, cal conjugate complex inobatwa ne anti-cal coating McAb pa membrane uye inoumba "anti-cal coating McAb-cal-colloidal gold yakanyorwa anti-cal McAb", colored test band yakaonekwa panzvimbo yekuyedza. Kusimba kweruvara kwakabatana zvakanaka ne cal content. Negative sample haiburitse test band nekuda kwekushaikwa kwe colloidal gold conjugate cal complex. Hazvinei kuti cal iripo mu sample kana kwete, pane red stripe inoonekwa panzvimbo yekuyedza uye nzvimbo yekudzora mhando, iyo inoonekwa seyemhando yemukati yebhizinesi.

Maitiro Ekuyedza:

1. Bvisa chimuti chekuyedza, chakaiswa mumuenzaniso wetsvina, wobva wadzosera chimuti chekuyedza, wochisunga zvakasimba wozunza zvakanaka, wodzokorora chiito katatu. Kana uchishandisa chimuti chekuyedza, tora sampuli yetsvina inosvika 50mg, woisa muchubhu yemuenzaniso wetsvina ine sampuli yekuyedza, wobva wasunga zvakasimba.

2. Shandisai nzira yekubvisa tsvina kubva muropa, torai nzira yetsvina yakatetepa kubva kumurwere ane manyoka, mobva mawedzera madonhwe matatu (anenge 100uL) muchubhu yetsvina mozunza zvakanaka, moisa parutivi.

3. Bvisa kadhi rekuyedza kubva mubhegi refoil, riise patafura yakaenzana worimaka.

4. Bvisa chivharo kubva pachubhu yemuenzaniso worasa madonhwe maviri ekutanga akaderedzwa mvura, wedzera madonhwe mana (anenge 100uL) pasina bubble diluted sample vertically wobva waisa zvishoma nezvishoma mutsime remuenzaniso wekadhi rine dispette yakapihwa, tanga nguva.

5. Mhedzisiro yacho inofanira kuverengwa mukati memaminitsi gumi kusvika gumi nemashanu, uye haichashandi mushure memaminitsi gumi nemashanu