ISISHWANKATHELO

IVithamini D yivithamini kwaye ikwayi-hormone ye-steroid, ikakhulu equka i-VD2 kunye ne-VD3, eyakhiwe ngendlela efanayo kakhulu. IVithamini D3 kunye ne-D2 ziguqulwa zibe yi-25 hydroxyl vitamin D (kuquka i-25-dihydroxyl vitamin D3 kunye ne-D2). I-25-(OH) VD emzimbeni womntu, ulwakhiwo oluzinzileyo, uxinaniso oluphezulu. I-25-(OH) VD ibonisa isixa siphela sevithamini D, kunye nokukwazi ukuguqula ivithamin D, ngoko ke i-25-(OH)VD ithathwa njengeyona nto ibalaseleyo yokuvavanya inqanaba levithamin D. I-Diagnostic Kit isekelwe kwi-immunochromatography kwaye inokunika isiphumo kwimizuzu eli-15.

UMGAQO WENKQUBO

I-membrane yesixhobo sovavanyo igqunywe nge-conjugate ye-BSA kunye ne-25-(OH)VD kwindawo yovavanyo kunye ne-antibody yebhokhwe ye-IgG antibody kwindawo yokulawula. I-Marker pad igqunywe nge-antibody ye-fluorescence mark anti-25-(OH)VD kunye ne-IgG yomvundla kwangaphambili. Xa kuvavanywa isampuli, i-25-(OH)VD kwisampuli idityaniswa ne-antibody ye-fluorescence mark anti-25-(OH)VD, kwaye yenze umxube wokuzikhusela. Phantsi kwesenzo se-immunochromatography, ukuhamba okuntsonkothileyo kwicala lephepha elifunxayo, xa i-complex idlule kwindawo yovavanyo, i-free fluorescent marker iya kudityaniswa ne-25-(OH)VD kwi-membrane. Uxinzelelo lwe-25-(OH)VD lulwalamano olubi lwesignali ye-fluorescence, kwaye uxinzelelo lwe-25-(OH)VD kwisampuli lunokubonwa ngovavanyo lwe-fluorescence immunoassay.