UMGAQO

I-membrane yesixhobo sovavanyo igqunywe nge-antibody ye-Hp-Ab kwindawo yovavanyo kunye ne-antibody ye-IgG yebhokhwe kwindawo yokulawula. I-Lable pad igqunywe nge-fluorescence ebhalwe nge-anti Hp-Ag kunye ne-IgG yerabbit kwangaphambili. Xa kuvavanywa isampuli echanekileyo, i-Hp-Ab kwisampuli idibana ne-fluorescence ebhalwe nge-anti Hp-Ag, kwaye yenze umxube we-immune. Phantsi kwesenzo se-immunochromatography, i-complex flow kwicala lephepha elifunxayo. Xa i-complex idlule kwindawo yovavanyo, idibene ne-anti Hp-Ag coating antibody, yenza i-complex entsha. Ukuba i-negative, akukho antibody ye-HP kwisampuli, ukuze i-immune complexes ingakwazi ukwakheka, akuyi kubakho mgca obomvu kwindawo yokufumanisa (T). Umgca obomvu ngumgangatho obonakala kwindawo yokulawula umgangatho (C) wokugweba ukuba kukho iisampulu ezaneleyo kunye nokuba inkqubo ye-chromatography iqhelekile na. Ikwasetyenziswa njengomgangatho wolawulo lwangaphakathi lwee-reagents.

Inkqubo yoVavanyo:

Nceda ufunde iphakheji engezantsi ngaphambi kokuba uyivavanye.

1. Khupha ikhadi lovavanyo kwingxowa yefoyile, ulibeke etafileni elinganayo uze uliphawule.

2. Yongeza amathontsi ama-2 esampuli yeserum okanye yeplasma (okanye amathontsi ama-3 esampuli yegazi elipheleleyo/yeminwe) ukuze uthambe isampuli yekhadi ene-dispette enikiweyo, uze wongeze ithontsi eli-1 lesampuli yokunyibilikisa, uze uqalise ixesha.

3. Linda ubuncinane imizuzu eli-10-15 uze ufunde iziphumo emva kwemizuzu eli-10-15. Iziphumo azisebenzi emva kwemizuzu eli-15.