UMGAQO

I-strip ine-anti-cal coating McAb kwindawo yovavanyo kunye ne-anti-rabbit IgG antibody yebhokhwe kwindawo yokulawula, ebotshelelwe kwi-membrane chromatography kwangaphambili. I-Lable pad igqunywe ngegolide ye-colloidal ebhalwe i-anti-cal McAb kunye ne-colloidal gold ebhalwe i-rabbit IgG antibody kwangaphambili. Xa kuvavanywa isampuli elungileyo, i-cal kwisampuli ihlanganiswe negolide ye-colloidal ebhalwe i-anti-cal McAb, kwaye yenze i-immune complex, njengoko ivunyelwe ukuba ihambe ecaleni kwe-test strip, i-cal conjugate complex ibanjwa yi-anti-cal coating McAb kwi-membrane kwaye yenze i-complex ethi "anti-cal coating McAb-cal-colloidal gold ebhalwe i-anti-cal McAb", ibhendi yovavanyo enemibala yavela kwindawo yovavanyo. Ubungakanani bombala buhambelana kakuhle nomxholo we-cal. Isampuli engalunganga ayivelisi ibhendi yovavanyo ngenxa yokungabikho kwe-colloidal gold conjugate cal complex. Nokuba i-cal ikhona kwisampuli okanye ayikho, kukho umgca obomvu obonakalayo kwindawo yokujonga kunye nommandla wolawulo lomgangatho, othathwa njengemigangatho yangaphakathi yeshishini yomgangatho.

Inkqubo yoVavanyo:

1. Khupha intonga yokuvavanya, efakwe kwisampuli yendle, uze uyibuyisele intonga yokuvavanya, uyiqinise uze uyishukumise kakuhle, uphinde wenze loo nto izihlandlo ezi-3. Okanye usebenzisa intonga yokuvavanya ukhethe isampuli yendle engama-50mg, uze uyifake kwityhubhu yesampuli yendle equlethe isampuli yokunyibilikisa, uze uyijije ngokuqinileyo.

2. Sebenzisa isampulu yepayipi elahlwayo, thatha isampuli yelindle elincinci kumguli orhudayo, uze wongeze amathontsi amathathu (malunga ne-100uL) kwityhubhu yokuvavanya ilindle uze ushukumise kakuhle, ubeke ecaleni.

3.Khupha ikhadi lovavanyo kwingxowa yefoyile, ulibeke etafileni elinganayo uze uliphawule.

4. Susa isiciko kwityhubhu yesampulu uze ulahle amathontsi amabini okuqala esampuli exutyiweyo, yongeza amathontsi ama-4 (malunga ne-100uL) isampuli exutyiweyo engaxutyiweyo ngokuthe nkqo uze kancinci ungene kwisampuli equlathe ikhadi nge-dispette enikiweyo, ixesha lokuqala.

5. Isiphumo kufuneka sifundwe kwimizuzu eli-10-15, kwaye asisebenzi emva kwemizuzu eli-15