Ikhithi Yokuxilonga ye-Procalcitonin

(ukuhlolwa kwe-fluorescence immunochromatographic)

Ukusetshenziswa kokuxilonga ngaphakathi kwesibeletho kuphela

Sicela ufunde lesi sithasiselo sephakheji ngokucophelela ngaphambi kokusebenzisa futhi ulandele imiyalelo ngokucophelela. Ukuthembeka kwemiphumela yokuhlola akunakuqinisekiswa uma kukhona ukuphambuka emiyalweni ekulesi sithasiselo sephakheji.

UKUSETSHENZISWA OKUHLOSIWE

Ikhithi Yokuxilonga ye-Procalcitonin (i-fluorescence immunochromatographic assay) iyi-fluorescence immunochromatographic assay yokuthola inani le-Procalcitonin (PCT) ku-serum noma ku-plasma yomuntu, isetshenziselwa ukuxilongwa okusizayo kokutheleleka ngamagciwane kanye ne-sepsis. Wonke amasampula amahle kumele aqinisekiswe ngezinye izindlela. Lokhu kuhlolwa kuhloselwe ukusetshenziswa kochwepheshe bezempilo kuphela.

ISIFINYEZO

I-Procalcitonin yakhiwe ngama-amino acid angu-116 kanti isisindo sayo sama-molecule singama-12.7KD. I-PCT ivezwa ngamaseli e-neuroendocrine futhi ihlukaniswe ngama-enzyme ibe yi-calcitonin (engavuthiwe), i-peptide eqeda i-carboxy, kanye ne-peptide eqeda i-amino. Abantu abaphilile banenani elincane le-PCT egazini labo, elingakhuliswa kakhulu ngemva kokutheleleka ngamagciwane. Lapho kwenzeka i-sepsis emzimbeni, izicubu eziningi zingaveza i-PCT, ngakho-ke i-PCT ingasetshenziswa njengesibonakaliso sokubikezela i-sepsis. Kwabanye abaguli abanokutheleleka okuvuvukalayo, i-PCT ingasetshenziswa njengesibonakaliso sokukhethwa kwama-antibiotic kanye nokwahlulela kokusebenza kahle.

ISIMISO SENQUBO

Ulwelwesi lwedivayisi yokuhlola lumbozwe nge-antibody ye-PCT esifundeni sokuhlola kanye ne-antibody yembuzi ye-anti rabbit IgG esifundeni sokulawula. Iphedi yelebula imbozwe nge-antibody ye-PCT ebhalwe nge-fluorescence kanye ne-IgG yenogwaja kusengaphambili. Lapho kuhlolwa isampula enhle, i-antigen ye-PCT esibonelweni ihlangana ne-antibody ye-antiPCT ebhalwe nge-fluorescence, bese yakha ingxube ye-immune. Ngaphansi kwesenzo se-immunochromatography, ukugeleza okuyinkimbinkimbi ohlangothini lwephepha elimunca, lapho i-complex idlula esifundeni sokuhlola, ihlanganiswe ne-antibody ye-antiPCT coating, yakha i-complex entsha. Izinga le-PCT lihlotshaniswa kahle nesignali ye-fluorescence, futhi ukuhlushwa kwe-PCT esibonelweni kungatholakala nge-fluorescence immunoassay assay.