ISIMISO

I-membrane yedivayisi yokuhlola imbozwe nge-conjugate ye-BSA kanye ne-25-(OH)VD esifundeni sokuhlola kanye ne-antibody yembuzi yokulwa nonogwaja IgG esifundeni sokulawula. Iphedi yomaka imbozwe nge-antibody ye-fluorescence mark anti 25-(OH)VD kanye ne-IgG yonogwaja kusengaphambili. Lapho kuhlolwa isampula, i-25-(OH)VD kusampula ihlangana ne-antibody ye-antibody ye-fluorescence mark anti 25-(OH)VD, bese yakha ingxube yokuzivikela komzimba. Ngaphansi kwesenzo se-immunochromatography, ukugeleza okuyinkimbinkimbi kuya ohlangothini lwephepha elimuncayo, lapho i-complex idlula esifundeni sokuhlola, i-free fluorescent marker izohlanganiswa ne-25-(OH)VD ku-membrane. Ukuhlushwa kwe-25-(OH)VD kuwukuxhumana okungekuhle kwesignali ye-fluorescence, futhi ukuhlushwa kwe-25-(OH)VD kusampula kungatholakala nge-fluorescence immunoassay assay.

Inqubo Yokuhlola:

Sicela ufunde incwadi yemiyalelo yokusebenza kwethuluzi kanye nephakheji ngaphambi kokuhlola.

1. Beka eceleni wonke ama-reagent namasampula endaweni yokushisa yegumbi.
2. Vula i-Portable Immune Analyzer (WIZ-A101), faka ukungena ngemvume kwephasiwedi ye-akhawunti ngokwendlela yokusebenza kwethuluzi, bese ufaka isikhombikubona sokuthola.
3. Skena ikhodi yokuqinisekisa ukuze uqinisekise into yokuhlola.
4. Khipha ikhadi lokuhlola esikhwameni se-foil.
5. Faka ikhadi lokuhlola embobeni yekhadi, skena ikhodi ye-QR, bese unquma into yokuhlolwa.
6. Faka isampula ye-serum noma i-plasma engu-15μL esixazululweni, bese uxuba kahle
7. Engeza ingxube engu-80μL ukuze uhlole umthombo wekhadi.
8. Chofoza inkinobho ethi "ukuhlolwa okujwayelekile", ngemva kwemizuzu eyi-15, ithuluzi lizobona ngokuzenzakalelayo ikhadi lokuhlola, lingafunda imiphumela esikrinini sokubonisa sethuluzi, bese liqopha/liphrinta imiphumela yokuhlolwa.
9. Bheka imiyalelo ye-Portable Immune Analyzer (WIZ-A101).