KA'IDA

Wannan tsiri yana da antigen mai rufewa na MP-Ag a yankin gwaji da kuma antibody mai hana akuya IgG a yankin sarrafawa, wanda aka manne shi da membrane chromatography a gaba. Ana shafa murfin Lable da colloidal zinariya mai lakabin IgM McAb a gaba. Lokacin gwada samfurin tabbatacce, MP-IgM a cikin samfurin yana haɗuwa da colloidal zinariya mai lakabin linzamin kwamfuta da anti human IgM McAb, kuma yana samar da hadaddun garkuwar jiki. A ƙarƙashin aikin immunochromatography, hadaddun da samfurin da ke cikin membrane na nitrocellulose suna gudana zuwa ga takardar sha, lokacin da hadaddun ya wuce yankin gwaji, ya haɗu da antigen mai rufewa na MP-Ag, yana samar da hadaddun "MP-Ag coating antigen-MP-IgM-colloidal zinariya mai lakabin linzamin kwamfuta da anti human IgM McAb", rukunin gwaji mai launi ya bayyana a yankin gwaji. Samfurin mara kyau ba ya samar da rukunin gwaji saboda ƙarancin hadaddun garkuwar jiki. Ko da MP-IgM yana cikin samfurin ko a'a, akwai layin ja a yankin sarrafa inganci, wanda ake ɗauka a matsayin ƙa'idodin inganci na cikin gida na kasuwanci.

Tsarin Gwaji:

Tsarin gwajin WIZ-A101 yana duba umarnin na'urar nazarin garkuwar jiki mai ɗaukuwa. Tsarin gwajin gani shine kamar haka:

1. A ajiye dukkan sinadaran da aka haɗa da samfuran a zafin ɗaki.
2. Cire katin gwaji daga jakar foil, sanya shi a kan teburin matakin sannan ka yi masa alama.
3. A ƙara samfurin jini na 10μL ko na plasma ko kuma cikakken jinin 20μL don yin gwajin rijiyar katin tare da dispette da aka bayar, sannan a ƙara 100μL (kimanin digo 2-3) na samfurin diluent, sannan a fara lokacin.
4. Jira aƙalla mintuna 10-15 sannan ka karanta sakamakon, sakamakon ba shi da inganci bayan mintuna 15.