UMGAQO

Umtya une-MP-Ag yokugquma i-antigen kwindawo yovavanyo kunye nebhokhwe ye-anti-mouse ye-IgG ye-antibody kwindawo yolawulo, ebotshelelwe kwi-membrane chromatography kwangaphambili.Iphedi yelebhile iqatywe ngegolide ecolloidal ebhalwe imouse-anti human IgM McAb kwangaphambili.Xa kuvavanywa isampulu eqinisekileyo, iMP-IgM kwisampulu idibanisa negolide ye-colloidal ebhalwe i-mouse-anti human IgM McAb, kwaye yenza i-immune complex.Ngaphantsi kwesenzo se-immunochromatography, i-complex kunye nesampulu yangaphakathi ye-nitrocellulose inwebu ihamba isiya kwicala lephepha elifunxayo, xa i-complex iphumelele ummandla wovavanyo, yadityaniswa ne-MP-Ag yokwambathisa i-antigen, yenze “i-MP-Ag yokwambathisa i-antigen-MP. -IgM-colloidal yegolide ebhalwe imouse-anti human IgM McAb” complex, ibhendi yovavanyo enemibala yavela kwindawo yovavanyo.Isampulu engalunganga ayivelisi ibhendi yovavanyo ngenxa yokusilela kwe-immune complex.Nokuba iMP-IgM ikhona kwisampulu okanye ayikho, kukho umgca obomvu ovela kummandla wolawulo lomgangatho, othathwa njengomgangatho wemigangatho yeshishini langaphakathi.

Inkqubo yoVavanyo:

Inkqubo yovavanyo ye-WIZ-A101 bona umyalelo we-immune analyzer ephathekayo.Inkqubo yovavanyo olubonakalayo lulandelayo:

1. Beka eceleni zonke ii-reagents kunye neesampuli kwiqondo lokushisa.
2. Khupha ikhadi lovavanyo kwibhegi yefoyile, uyibeke kwitafile yenqanaba kwaye uyiphawule.
3. Yongeza i-10μL ye-serum okanye isampuli yeplasma okanye i-20μL yesampulu yegazi epheleleyo kwisampulu kakuhle yekhadi kunye ne-dispette enikeziweyo, uze udibanise i-100μL (malunga ne-2-3 yehla) isampuli ye-diluent, qalisa ixesha.
4. Lindela ubuncinci bemizuzu eyi-10-15 kwaye ufunde umphumo, umphumo awuvumelekile emva kwemizuzu eyi-15.