ỤKPỤRỤ

Eriri ahụ nwere antigen mkpuchi MP-Ag na mpaghara nnwale na antibody mgbochi òké ewu na mpaghara njikwa, nke a na-ejikọ na membrane chromatography tupu oge eruo. A na-eji colloidal gold labeled mouse-anti human IgM McAb kpuchie pad Lable tupu oge eruo. Mgbe a na-anwale ihe nlele dị mma, MP-IgM dị na ihe nlele ahụ na-ejikọta ya na colloidal gold labeled mouse-anti human IgM McAb, ma mepụta ihe mgbochi ahụ. N'okpuru ọrụ nke immunochromatography, ihe mgbagwoju anya na ihe nlele dị n'ime membrane nitrocellulose na-asọgharị n'akụkụ akwụkwọ na-amịkọrọ ihe, mgbe ihe mgbagwoju anya gafere mpaghara nnwale ahụ, ọ jikọtara ya na antigen mkpuchi MP-Ag, na-emepụta ihe mgbagwoju anya "MP-Ag mkpuchi antigen-MP-IgM-colloidal gold labeled mouse-anti human IgM McAb", otu eriri nnwale agba pụtara na mpaghara nnwale ahụ. Ihe nlele na-adịghị mma anaghị emepụta eriri nnwale n'ihi enweghị ihe mgbochi ahụ. N'agbanyeghị na MP-IgM dị na ihe nlele ahụ ma ọ bụ na ọ nọghị, enwere eriri uhie na-apụta na mpaghara njikwa mma, nke a na-ewere dị ka ụkpụrụ ụlọ ọrụ dị n'ime mma.

Usoro Ule:

Usoro nnwale WIZ-A101 na-ahụ ntuziaka nke ihe nyocha mgbochi ọrịa ebugharị. Usoro nnwale anya bụ dịka ndị a:

1. Debe ihe niile e ji eme ihe na ihe nlele n'akụkụ okpomọkụ ụlọ.
2. Wepụ kaadị ule ahụ n'ime akpa foil ahụ, tinye ya na tebụl dị larịị wee tinye akara na ya.
3. Tinye ihe nlele serum ma ọ bụ plasma 10μL ma ọ bụ ihe nlele ọbara dum 20μL iji nwalee nke ọma nke kaadị ahụ na ihe nlele enyere, wee tinye ihe nlele 100μL (ihe dị ka ntapụ 2-3) nke ihe nlele ahụ, malite oge.
4. Chere opekata mpe nkeji iri ruo iri na ise wee gụọ nsonaazụ ya, nsonaazụ ya agaghị adị irè mgbe nkeji iri na ise gachara.