ỤKPỤRỤ

Eriri a nwere mkpuchi mgbochi cal McAb na mpaghara nnwale na mgbochi IgG nke ewu na mpaghara njikwa, nke a na-ejikọ na membrane chromatography tupu oge eruo. A na-ekpuchi paadị lable site na colloidal gold labeled anti cal McAb na colloidal gold labeled IgG antibody tupu oge eruo. Mgbe a na-anwale ihe nlele dị mma, a na-ejikọta cal dị na ihe nlele ahụ na colloidal gold labeled anti cal McAb, ma mepụta ihe mgbochi ahụ, ebe ọ bụ na a na-ekwe ka ọ kwaga n'akụkụ mpempe ule ahụ, a na-ejide ihe mgbagwoju cal conjugate site na mkpuchi anti cal McAb na akpụkpọ ahụ ma mepụta "mkpuchi anti cal McAb" nke McAb-cal-colloidal gold labeled anti cal McAb, eriri ule agba pụtara na mpaghara nnwale ahụ. Ike agba ahụ na-ejikọta nke ọma na ọdịnaya cal. Ihe nlele na-adịghị mma anaghị emepụta eriri ule n'ihi enweghị colloidal gold conjugate cal complex. N'agbanyeghị cal dị na ihe nlele ahụ ma ọ bụ na ọ dịghị, enwere eriri uhie na-apụta na mpaghara ntụaka na mpaghara njikwa mma, nke a na-ewere dị ka ụkpụrụ ụlọ ọrụ dị n'ime.

Usoro Ule:

1. Wepụ ihe nlele ahụ, tinye ya n'ime ihe nlele nsị ahụ, wee tinye ihe nlele ahụ azụ, kpụchie ya nke ọma ma maa jijiji nke ọma, megharịa ihe ahụ ugboro atọ. Ma ọ bụ jiri ihe nlele ahụ họrọ ihe nlele nsị ihe dị ka 50mg, tinye ya n'ime tube ihe nlele nsị nke nwere ihe nlele ahụ, wee kpụchie ya nke ọma.

2. Jiri ihe nlele pipette a na-atụfu atụfu, were ihe nlele nsị dị gịrịgịrị n'aka onye ọrịa afọ ọsịsa, wee tinye ntapụ atọ (ihe dị ka 100uL) n'ime tube ihe nlele nsị wee maa jijiji nke ọma, debe ya n'akụkụ.

3. Wepụ kaadị ule ahụ n'ime akpa foil ahụ, tinye ya na tebụl dị larịị wee tinye akara na ya.

4. Wepụ mkpuchi ahụ n'ime tube ihe nlele ahụ wee tụfuo mmiri abụọ mbụ ahụ a gwakọtara agwakọta, tinye mmiri 4. (ihe dị ka 100uL) nke a gwakọtara agwakọta nke ọma ma jiri nwayọọ nwayọọ banye n'ime olulu mmiri nke kaadị ahụ na ihe e nyere ya, malite oge.

5. A ga-agụ ihe a ga-esi na ya pụta n'ime nkeji iri ruo iri na ise, ọ gaghịkwa adị irè mgbe nkeji iri na ise gachara.