Kete Tātaritanga mōpūmua C-reactive tairongo nui

(whakamātautau ārai-ira-whakaahua-whakaahua)

Mō te whakamahinga tātaritanga in vitro anake

Pānuihia tēnei tāpiringa i mua i te whakamahinga, ā, me whai pūmau i ngā tohutohu. Kāore e taea te whakamana i te pono o ngā hua whakamātautau mēnā he rerekētanga mai i ngā tohutohu kei roto i tēnei tāpiringa.

TE WHAKAMAHINGA I WHAKATAURANGIA

Ko te Kete Tātaritanga mō te pūmua C-reactive tairongo nui (whakamātautau immunochromatographic fluorescence) he whakamātautau immunochromatographic fluorescence mō te kimi i te nui o te pūmua C-reactive (CRP) i roto i te toto tangata /plasma / toto katoa. He tohu kore-motuhake mō te mumura. Me whakaū te tauira pai katoa mā ētahi atu tikanga. Ko tēnei whakamātautau he mea hanga mō te whakamahinga hauora anake.

WHAKARĀPOPOTONGA

Ko te pūmua C-reactive he pūmua wāhanga tere i hangaia e te whakaoho lymphokine o te ate me ngā pūtau epithelial. Kei roto i te serum tangata, te wai cerebrospinal, te wai pleural me te wai puku, me ētahi atu, ā, he wāhanga o te pūnaha ārai mate kore-motuhake. 6-8 hāora i muri i te putanga mai o te mate kitakita, ka tīmata te piki o te CRP, 24-48 hāora ka tae ki te tihi, ā, ka taea e te uara tihi te eke ki ngā rau o ngā wā o te noa. I muri i te whakakorenga o te mate, ka heke tere te CRP, ā, ka hoki ki te noa i roto i te wiki kotahi. Heoi, kāore te CRP e tino piki ake i roto i te take o te mate huaketo, e whakarato ana i te pūtake mō te tautuhi i ngā momo mate o te mate wawe, ā, he taputapu mō te tautuhi i ngā mate huaketo, kitakita rānei.

TE MĀTĀPONO O TE TIKANGA

Kua pania te kiriuhi o te taputapu whakamātautau ki te paturopi anti-CRP i te rohe whakamātautau, me te paturopi anti-rāpeti koati IgG i te rohe whakahaere. Kua pania te papa tapanga ki te paturopi anti-CRP me te IgG rāpeti kua tohua ki te fluorescence i mua i te whakamātautau. I te whakamātautau i te tauira pai, ka honoa te antigen CRP i roto i te tauira ki te paturopi anti-CRP kua tohua ki te fluorescence, ka hanga he ranunga ārai mate. I raro i te mahi a te immunochromatography, ka rere te matatini i te ahunga o te pepa mimiti, ina paahitia e te matatini te rohe whakamātautau, ka honoa ki te paturopi paninga anti-CRP, ka hanga he matatini hou. He hononga pai te taumata CRP ki te tohu fluorescence, ā, ka taea te kite i te kukū o te CRP i roto i te tauira mā te whakamātautau fluorescence immunoassay.

NGĀ RĀWENI ME NGĀ RAUEMI I TUKUNA

Ngā wāhanga mōkihi 25T：

Kāri whakamātautau takitahi, he mea takai ki te pepa konutea me te whakamaroke 25T

Ngā wairewa tauira 25T

Tāpiringa mōkihi 1

NGĀ RAUEMI E HIAHIATIA ANA ENGARI KĀORE I HOATU

Ipu kohinga tauira, taima

TE KOHINGA TAUIRA ME TE ROKIROKI

1. Ko ngā tauira i whakamatautauria he toto toto, he plasma heparin anticoagulant, he plasma EDTA anticoagulant rānei.
2. E ai ki ngā tikanga paerewa, kohia he tauira. Ka taea te pupuri i te tauira toto, i te tauira plasma rānei ki roto i te pouaka whakamātao i te 2-8℃ mō ngā rā e 7, ā, ka taea te pupuri i te tauira toto katoa ki raro i te -15°C mō ngā marama e 6. Ka taea te pupuri i te tauira toto katoa ki roto i te pouaka whakamātao i te 2-8℃ mō ngā rā e 3.
3. Ka karohia e ngā tauira katoa ngā huringa tio-rewa.