WHAKARĀPOPOTONGA

He huaora te Huaora D, ā, he homoni steroid anō hoki, ko te nuinga kei roto ko te VD2 me te VD3, ā, he tino ōrite te hanganga. Ka hurihia te Huaora D3 me te D2 ki te 25 hydroxyl huaora D (tae atu ki te 25-dihydroxyl huaora D3 me te D2). He 25-(OH) VD i roto i te tinana tangata, he hanganga pumau, he kukū teitei. Ko te 25-(OH) VD e whakaata ana i te nui katoa o te huaora D, me te kaha hurihanga o te huaora D, nō reira e kiia ana ko te 25-(OH) VD te tohu pai rawa atu mō te aromatawai i te taumata o te huaora D. Ko te Kete Tātaritanga e hangai ana ki te immunochromatography, ā, ka taea te whakaputa hua i roto i te 15 meneti.

TE MĀTĀPONO O TE TIKANGA

Kua pania te kiriuhi o te taputapu whakamātautau ki te hononga o te BSA me te 25-(OH)VD i te rohe whakamātautau me te paturopi IgG ārai rāpeti koati i te rohe whakahaere. Kua pania ngā papa tohu ki te tohu fluorescence ārai rāpeti 25-(OH)VD me te IgG rāpeti i mua. I te whakamātautau i te tauira, ka honoa te 25-(OH)VD i roto i te tauira ki te paturopi ārai rāpeti kua tohua ki te fluorescence, ā, ka hanga he ranunga ārai mate. I raro i te mahi a te immunochromatography, ka rere te matatini i te ahunga o te pepa mimiti, ina paahitia e te matatini te rohe whakamātautau, ka honoa te tohu fluorescence kore utu ki te 25-(OH)VD i runga i te kiriuhi. Ko te kukū o te 25-(OH)VD he hononga kino mō te tohu fluorescence, ā, ka taea te kite i te kukū o te 25-(OH)VD i roto i te tauira mā te whakamātautau immunoassay fluorescence.