KAUPAPA

Ko te Huaora D he huaora, he homoni steroid ano hoki, ko te nuinga ko te VD2 me te VD3, he tino rite te hanganga. Ka hurihia te Huaora D3 me te D2 ki te 25 hydroxyl vitamin D (tae atu ki te 25-dihydroxyl vitamin D3 me D2). 25-(OH) VD i roto i te tinana tangata, struction pūmau, kukū tiketike. Ko te 25-(OH) VD e whakaatu ana i te tapeke o te huaora D , me te kaha hurihanga o te huaora D, na ko te 25-(OH)VD ka whakaarohia ko te tohu pai rawa atu mo te aromatawai i te taumata o te huaora D. Ko te Kete Tohunga i runga i te immunochromatography ka taea te whakaputa hua i roto i te 15 meneti.

MĀTĀPONO O TE TIKANGA

Ko te kiriuhi o te taputapu whakamatautau ka pania ki te whakakotahitanga o te BSA me te 25-(OH)VD i te rohe whakamatautau me te koati anti rabbit IgG antibody i runga i te rohe whakahaere. Ka pania te papa tohu ki te tohu fluorescence anti 25-(OH)VD antibody me te IgG rapeti i mua. I te wa e whakamatautau ana i te tauira, 25-(OH)VD i roto i te tauira ka whakakotahi me te rewharewha kua tohua he anti 25-(OH)VD antibody, ka hanga i te ranunga aukati. I raro i te mahi o te immunochromatography, te rere matatini i roto i te ahunga o te pepa absorbent, ka pahemo te matatini i te rohe whakamātautau, Ko te tohu fluorescent free ka hono ki te 25- (OH) VD i runga i te membrane.Ko te kukū o 25- (OH) VD he hononga kino mo te tohu fluorescence, me te kukū o te 25- (OH)VD i roto i te tauira ka taea te kitea e te rewharewha.