MFUNDO

Chingwecho chili ndi antigen ya MP-Ag coating pa dera loyesera ndi antibody ya mbewa ya IgG yolimbana ndi mbewa yomwe ili pagawo lolamulira, yomwe imalumikizidwa ku membrane chromatography pasadakhale. Lable pad imakutidwa ndi colloidal gold labeld mouse-anti human IgM McAb pasadakhale. Poyesa chitsanzo chabwino, MP-IgM yomwe ili mu chitsanzo imasakanikirana ndi colloidal gold labeld mouse-anti human IgM McAb, ndikupanga immune complex. Pogwiritsa ntchito immunochromatography, complex ndi chitsanzo mkati mwa nitrocellulose membrane zimayenda molunjika ku pepala loyamwa, pamene complex idadutsa dera loyesera, idaphatikizana ndi MP-Ag coating antigen, ndikupanga "MP-Ag coating antigen-MP-IgM-colloidal gold labeld mouse-anti human IgM McAb", gulu loyesera lamitundu lidawonekera pagawo loyesera. Chitsanzo choyipa sichipanga gulu loyesera chifukwa cha immune complex yofooka. Kaya MP-IgM ilipo mu chitsanzo kapena ayi, pali mzere wofiira womwe umawonekera pagawo lowongolera khalidwe, lomwe limawonedwa ngati miyezo yamkati yamakampani abwino.

Njira Yoyesera:

Njira yoyesera ya WIZ-A101 onani malangizo a chowunikira chitetezo chamthupi chonyamulika. Njira yoyesera yowonera ndi iyi:

1. Ikani pambali zinthu zonse zoyeretsera ndi zitsanzo kutentha kwa chipinda.
2. Tulutsani khadi loyesera m'thumba la foil, liyikeni patebulo lolingana ndipo liyikeni chizindikiro.
3. Onjezani 10μL seramu kapena plasma chitsanzo kapena 20μL chitsanzo cha magazi onse kuti muyese chitsime cha khadi ndi dispette yoperekedwa, kenako onjezerani 100μL (pafupifupi madontho 2-3) chitsanzo chosungunula, nthawi yoyambira.
4. Yembekezerani kwa mphindi zosachepera 10-15 ndipo muwerenge zotsatira zake, zotsatira zake zimakhala zosavomerezeka patatha mphindi 15.