MFUNDO

Mzerewu uli ndi antigen yotchinga ya MP-Ag pamalo oyesera ndi anti-mouse IgG antibody pagawo lowongolera, yomwe imamangiriridwa ku membrane chromatography pasadakhale. Lable pad imakutidwa ndi golide wa colloidal wolembedwa mbewa-anti human IgM McAb pasadakhale. Mukayesa zitsanzo zabwino, MP-IgM mu zitsanzo imaphatikizana ndi golide wa colloidal wotchedwa IgM McAb wa mbewa, ndikupanga chitetezo chamthupi. Pansi pa zochita za immunochromatography, zovuta ndi chitsanzo mkati mwa nitrocellulose nembanemba otaya malangizo a absorbent pepala, pamene zovuta anapambana mayeso dera, izo pamodzi ndi MP-Ag ❖ kuyanika antigen, n'kupanga "MP-Ag ❖ kuyanika antigen-MP-IgM-colloidal golide otchedwa mbewa-otsutsa anthu IgM McAb" band anaonekera pa mtundu mayeso dera mayeso. Chitsanzo choyipa sichimapanga gulu loyesera chifukwa chosowa chitetezo chamthupi. Ziribe kanthu kuti MP-IgM ilipo mu zitsanzo kapena ayi, pali mizere yofiyira yomwe imawonekera pagawo lowongolera, lomwe limawonedwa ngati miyezo yamabizinesi amkati.

Njira Yoyesera:

Njira yoyesera ya WIZ-A101 iwona malangizo a chitetezo chamthupi chosanthula. Njira yoyeserera yowonera ili motere:

1. Ikani pambali ma reagents ndi zitsanzo ku kutentha kwa chipinda.
2. Tulutsani khadi loyesera mu thumba la zojambulazo, liyikeni pa tebulo lapamwamba ndikuyika chizindikiro.
3. Onjezani 10μL seramu kapena madzi a m'magazi kapena 20μL yamagazi athunthu kuti muyese bwino pakhadi lomwe lili ndi dispette, kenaka yikani 100μL (pafupifupi 2-3 dontho) chitsanzo chochepetsera, yambani nthawi.
4. Dikirani kwa mphindi zosachepera 10-15 ndikuwerenga zotsatira zake, zotsatira zake ndizosavomerezeka pakatha mphindi 15.